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Literature summary for 3.4.24.28 extracted from
- Refolding of the non-specific neutral protease from Bacillus stearothermophilus proceeds via an autoproteolytically sensitive intermediate (2010), Biophys. Chem., 147, 66-73.
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| G8C/N60C | thermostable variant of the non-specific neutral protease | Geobacillus stearothermophilus |
General Stability
| General Stability | Organism |
|---|---|
| native wild type and G8C/N60C enzymes are stable toward autoproteolysis and keep their structural integrity for several weeks | Geobacillus stearothermophilus |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 5 mM Ca2+ stabilizes the enzyme | Geobacillus stearothermophilus |  |
| Zn2+ | Zn2+ ions, although essential cofactors and exhibiting extremely high affinity, are without effect on the stability | Geobacillus stearothermophilus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Geobacillus stearothermophilus | - |
- |
- |
Renatured (Commentary)
| Renatured (Comment) | Organism |
|---|---|
| protease activity can be significantly regained if the completely unfolded enzyme is transferred from 8 M guanidine-HCl into native-like conditions (0.4 M guanidine-HCl) by dilution with buffer | Geobacillus stearothermophilus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-aminobenzoyl-Ala-Gly-Leu-Ala-4-nitrobenzylamide + H2O | - |
Geobacillus stearothermophilus | ? | - |
? | |
| casein + H2O | - |
Geobacillus stearothermophilus | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| non-specific neutral protease | - |
Geobacillus stearothermophilus |
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Release 2023.1 (January 2023)
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