Literature summary for 3.4.24.28 extracted from

Mansfeld, J.; Petermann, E.; Duerrschmidt, P.; Ulbrich-Hofmann, R.
The propeptide is not required to produce catalytically active neutral protease from Bacillus stearothermophilus (2005), Protein Expr. Purif., 39, 219-228.

Search for more literature for 3.4.24.28

Application

Application	Comment	Organism
synthesis	production of enzyme as an active mature enzyme in Escherichia coli in the absence of its prosequence. Expression of enzyme with N-terminal His-tag, which accumulates as inclusion bodies and renaturation after solubilization using guanidine hydrochloride	Geobacillus stearothermophilus

Organism

Organism	UniProt	Comment	Textmining
Geobacillus stearothermophilus	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
after expression in Escherichia coli with N-terminal His-tag	Geobacillus stearothermophilus

Renatured (Commentary)

Renatured (Comment)	Organism
renaturation of enzyme from inclusion bodies after solubilization using guanidine hydrochloride	Geobacillus stearothermophilus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
65.5	-	-	Geobacillus stearothermophilus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
casein + H2O	-	Geobacillus stearothermophilus	?	-	?

Synonyms

Synonyms	Comment	Organism
TLP-ste	-	Geobacillus stearothermophilus

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Release 2023.1 (January 2023)