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Literature summary for 3.4.24.28 extracted from

  • Narasaki, R.; Kuribayashi, H.; Shimizu, K.; Imamura, D.; Sato, T.; Hasumi, K.
    Bacillolysin MA, a novel bacterial metalloproteinase that produces angiostatin-like fragments from plasminogen and activates protease zymogens in the coagulation and fibrinolysis systems (2005), J. Biol. Chem., 280, 14278-14287.
    View publication on PubMed
Search for more literature for 3.4.24.28

Activating Compound

Activating Compound Comment Organism Structure
CoCl2 0.3 mM, 1.8fold increase in activity Priestia megaterium

Inhibitors

Inhibitors Comment Organism Structure
EDTA 1 mM, complete inhibition Priestia megaterium
additional information not inhibitory: 1 mM iodoacetic acid, 1 mM phenylmethylsulfonyl fluoride, 0.5 mM leupeptin, 0.5 mM pepstatin, 0.005 mM alpah2-plasmin inhibitor Priestia megaterium
Zn2+ 1 mM, 80% loss of activity Priestia megaterium

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.003
-
 plasminogen pH 7.4, 25°C Priestia megaterium

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35300
-
 x * 35300, SDS-PAGE Priestia megaterium

Organism

Organism UniProt Comment Textmining
Priestia megaterium
-
 A9542
-
Priestia megaterium A9542
-
 A9542
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-(2-furylacryloyl)-glycyl-L-leucine amide + H2O
-
 Priestia megaterium ?
-
 ?
3-(2-furylacryloyl)-glycyl-L-leucine amide + H2O
-
 Priestia megaterium A9542 ?
-
 ?
casein + H2O
-
 Priestia megaterium ?
-
 ?
casein + H2O
-
 Priestia megaterium A9542 ?
-
 ?
coagulation factor X + H2O
-
 Priestia megaterium ?
-
 ?
coagulation factor X + H2O
-
 Priestia megaterium A9542 ?
-
 ?
Gelatin + H2O
-
 Priestia megaterium ?
-
 ?
Gelatin + H2O
-
 Priestia megaterium A9542 ?
-
 ?
plasminogen + H2O cleavage mainly at S441-V442 Priestia megaterium angiostatin + mini-plasminogen
-
 ?
protein C + H2O
-
 Priestia megaterium ?
-
 ?
protein C + H2O
-
 Priestia megaterium A9542 ?
-
 ?
prothrombin + H2O
-
 Priestia megaterium thrombin + thrombin propeptide
-
 ?
prourokinase + H2O
-
 Priestia megaterium urokinase + urokinase propeptide
-
 ?

Subunits

Subunits Comment Organism
? x * 35300, SDS-PAGE Priestia megaterium

Synonyms

Synonyms Comment Organism
BL-MA
-
 Priestia megaterium

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 Priestia megaterium

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
25 60 stable Priestia megaterium
70
-
 20 min, 65% loss of activity Priestia megaterium

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.7
-
 plasminogen pH 7.4, 25°C Priestia megaterium

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
-
 Priestia megaterium

pH Stability

pH Stability pH Stability Maximum Comment Organism
4
-
 60 min, complete loss of activity Priestia megaterium
5 9 37°C, 60 min, stable Priestia megaterium
11.7
-
 60 min, complete loss of activity Priestia megaterium