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Literature summary for 3.4.24.27 extracted from

  • Ripp, S.; Turunen, P.; Minot, E.D.; Rowan, A.E.; Blank, K.G.
    Deciphering design principles of Foerster resonance energy transfer-based protease substrates thermolysin-like protease from Geobacillus stearothermophilus as a test case (2018), ACS Omega, 3, 4148-4156 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.24.27

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0024
-
 5,6-carboxyfluorescein-PAGLAC(Cy5)-NH2 pH 7.4, 30°C Geobacillus stearothermophilus
0.0116
-
 5,6-carboxyfluorescein-PVAGLAGC(Cy5)-NH2 pH 7.4, 30°C Geobacillus stearothermophilus
0.0176
-
 5,6-carboxyfluorescein-PSVAGLAGGC(Cy5)-NH2 pH 7.4, 30°C Geobacillus stearothermophilus
0.0533
-
 5,6-carboxyfluorescein-PAGLAC(Alexa Fluor 647)-NH2 pH 7.4, 30°C Geobacillus stearothermophilus
0.0776
-
 5,6-carboxyfluorescein-PSVAGLAGGC(Alexa Fluor 647)-NH2 pH 7.4, 30°C Geobacillus stearothermophilus

Organism

Organism UniProt Comment Textmining
Geobacillus stearothermophilus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5,6-carboxyfluorescein-PAGLAC(Alexa Fluor 647)-NH2 + H2O
-
 Geobacillus stearothermophilus 5,6-carboxyfluorescein-PAG + LAC(Alexa Fluor 647)-NH2
-
 ?
5,6-carboxyfluorescein-PAGLAC(Cy5)-NH2 + H2O
-
 Geobacillus stearothermophilus 5,6-carboxyfluorescein-PAG + LAC(Cy5)-NH2
-
 ?
5,6-carboxyfluorescein-PSVAGLAGGC(Alexa Fluor 647)-NH2 + H2O
-
 Geobacillus stearothermophilus 5,6-carboxyfluorescein-PSVAG + LAGGC(Alexa Fluor 647)-NH2
-
 ?
5,6-carboxyfluorescein-PSVAGLAGGC(Cy5)-NH2 + H2O
-
 Geobacillus stearothermophilus 5,6-carboxyfluorescein-PSVAG + LAGGC(Cy5)-NH2
-
 ?
5,6-carboxyfluorescein-PVAGLAGC(Cy5)-NH2 + H2O
-
 Geobacillus stearothermophilus 5,6-carboxyfluorescein-PVAG + LAGC(Cy5)-NH2
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.37
-
 5,6-carboxyfluorescein-PAGLAC(Cy5)-NH2 pH 7.4, 30°C Geobacillus stearothermophilus
0.7
-
 5,6-carboxyfluorescein-PAGLAC(Alexa Fluor 647)-NH2 pH 7.4, 30°C Geobacillus stearothermophilus
2.96
-
 5,6-carboxyfluorescein-PVAGLAGC(Cy5)-NH2 pH 7.4, 30°C Geobacillus stearothermophilus
5.7
-
 5,6-carboxyfluorescein-PSVAGLAGGC(Alexa Fluor 647)-NH2 pH 7.4, 30°C Geobacillus stearothermophilus
7.82
-
 5,6-carboxyfluorescein-PSVAGLAGGC(Cy5)-NH2 pH 7.4, 30°C Geobacillus stearothermophilus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
13.1
-
 5,6-carboxyfluorescein-PAGLAC(Alexa Fluor 647)-NH2 pH 7.4, 30°C Geobacillus stearothermophilus
73.5
-
 5,6-carboxyfluorescein-PSVAGLAGGC(Alexa Fluor 647)-NH2 pH 7.4, 30°C Geobacillus stearothermophilus
154.2
-
 5,6-carboxyfluorescein-PAGLAC(Cy5)-NH2 pH 7.4, 30°C Geobacillus stearothermophilus
255.2
-
 5,6-carboxyfluorescein-PVAGLAGC(Cy5)-NH2 pH 7.4, 30°C Geobacillus stearothermophilus
444.3
-
 5,6-carboxyfluorescein-PSVAGLAGGC(Cy5)-NH2 pH 7.4, 30°C Geobacillus stearothermophilus