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Literature summary for 3.4.24.27 extracted from

  • Yang, J.J.; van Wart, H.E.
    Kinetics of hydrolysis of dansyl peptide substrates by thermolysin: analysis of fluorescence changes and determination of steady-state kinetic parameters (1994), Biochemistry, 33, 6508-6515.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.08 0.09 Dansyl-Gly-Phe-Ala depending on assay method Bacillus thermoproteolyticus
0.2
-
dansyl-Ala-Ala-Phe-Ala
-
Bacillus thermoproteolyticus
0.3
-
dansyl-Gly-Phe-Phe
-
Bacillus thermoproteolyticus
0.62
-
Phe-Leu-Ala-NH(CH2)2NH-dansyl
-
Bacillus thermoproteolyticus
0.69
-
dansyl-Gly-Leu-Phe
-
Bacillus thermoproteolyticus
0.77
-
dansyl-Gly-Phe-Gly
-
Bacillus thermoproteolyticus
0.91
-
dansyl-Ala-Phe-Ala
-
Bacillus thermoproteolyticus
2
-
dansyl-Ala-Leu-Ala
-
Bacillus thermoproteolyticus
5
-
dansyl-Gly-Leu-Gly
-
Bacillus thermoproteolyticus
13
-
dansyl-Gly-Gly-Leu-Gly
-
Bacillus thermoproteolyticus

Organism

Organism UniProt Comment Textmining
Bacillus thermoproteolyticus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Dansyl peptides + H2O
-
Bacillus thermoproteolyticus ?
-
?
dansyl-Ala-Ala-Phe-Ala + H2O
-
Bacillus thermoproteolyticus ?
-
?
dansyl-Ala-Leu-Ala + H2O
-
Bacillus thermoproteolyticus ?
-
?
dansyl-Ala-Phe-Ala + H2O
-
Bacillus thermoproteolyticus ?
-
?
dansyl-Gly-Gly-Leu-Gly + H2O
-
Bacillus thermoproteolyticus ?
-
?
dansyl-Gly-Leu-Gly + H2O
-
Bacillus thermoproteolyticus ?
-
?
dansyl-Gly-Leu-Phe + H2O
-
Bacillus thermoproteolyticus ?
-
?
Dansyl-Gly-Phe-Ala + H2O
-
Bacillus thermoproteolyticus ?
-
?
dansyl-Gly-Phe-Gly + H2O
-
Bacillus thermoproteolyticus ?
-
?
dansyl-Gly-Phe-Phe + H2O
-
Bacillus thermoproteolyticus ?
-
?
Phe-Leu-Ala-NH(CH2)2NH-dansyl + H2O
-
Bacillus thermoproteolyticus ?
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.4 1.7 Dansyl-Gly-Phe-Ala depending on assay method Bacillus thermoproteolyticus