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Literature summary for 3.4.24.26 extracted from
- Characterization of an organic-solvent-stable elastase from Pseudomonas indica and its potential use in eggshell membrane hydrolysis (2019), Proc. Biochem., 85, 156-163 .
No PubMed abstract available
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| sodium sulfite | enhances hydrolysis of eggshell-membrane | Pseudomonas indica |  |
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | the enzyme can be applied to obtain bioactive soluble peptides from eggshell-membrane. Potential applicability of its peptides as functional food and cosmetic additives | Pseudomonas indica |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Pseudomonas indica |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| CH2ICOOH | 3 mM,88 % inhibition | Pseudomonas indica | |
| DTT | 3 mM, 24% inhibition | Pseudomonas indica | |
| EDTA | 3 mM, 14% inhibition | Pseudomonas indica | |
| phosphoramidon | 3 mM, 13% inhibition | Pseudomonas indica | |
| PMSF | 5 mM, 27% inhibition | Pseudomonas indica | |
| sodium sulfite | 3 mM, 94% inhibition | Pseudomonas indica | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Pseudomonas indica | - |
- |
Organic Solvent Stability
| Organic Solvent | Comment | Organism |
|---|---|---|
| 2-propanol | 25% (v/v), 1 week, stable | Pseudomonas indica |
| Acetone | 25% (v/v), 1 week, stable | Pseudomonas indica |
| DMSO | 25% (v/v), 1 week, stable | Pseudomonas indica |
| Ethanol | 25% (v/v), 1 week, stable | Pseudomonas indica |
| Ethyl acetate | 25% (v/v), 1 week, stable | Pseudomonas indica |
| hexane | 25% (v/v), 1 week, stable | Pseudomonas indica |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas indica | WP_084338031.1 | - |
- |
| Pseudomonas indica NBRC 103045 | WP_084338031.1 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Pseudomonas indica |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| culture fluid | - |
Pseudomonas indica | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| casein + H2O | - |
Pseudomonas indica | ? | - |
? | |
| casein + H2O | - |
Pseudomonas indica NBRC 103045 | ? | - |
? | |
| eggshell-membrane + H2O | the enzyme can be applied to obtain bioactive soluble peptides from eggshell-membrane | Pseudomonas indica | ? | - |
? | |
| eggshell-membrane + H2O | the enzyme can be applied to obtain bioactive soluble peptides from eggshell-membrane | Pseudomonas indica NBRC 103045 | ? | - |
? | |
| elasti-orcein + H2O | - |
Pseudomonas indica | ? | - |
? | |
| elasti-orcein + H2O | - |
Pseudomonas indica NBRC 103045 | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| LasB | - |
Pseudomonas indica |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
hydrolysis of eggshell-membrane | Pseudomonas indica |
| 55 | - |
casein hydrolysis | Pseudomonas indica |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 55 | stable | Pseudomonas indica |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | 9 | - |
Pseudomonas indica |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | 12 | stable | Pseudomonas indica |
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