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Literature summary for 3.4.24.26 extracted from
- High-level expression of pseudolysin, the extracellular elastase of Pseudomonas aeruginosa, in Escherichia coli and its purification (2015), Protein Expr. Purif., 113, 79-84.
Application
| Application | Comment | Organism |
|---|---|---|
| industry | pseudolysin is a biotechnologically important enzyme in the tanning industry | Pseudomonas aeruginosa |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene lasB, recombinant expression in Escherichia coli and in Pichia pastoris and secretion of the enzyme, large-scale expression of His-tagged enzyme under the control of the T7 promoter in Escherichia coli strain BL21(DE3) in inclusion bodies | Pseudomonas aeruginosa |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Pseudomonas aeruginosa | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Zn2+ | a zinc metalloprotease | Pseudomonas aeruginosa |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 34160 | - |
x * 34160, sequence calculation | Pseudomonas aeruginosa |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas aeruginosa | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| solubilized recombinant His-tagged enzyme from Escherichia coli cells by metal affinity chromatography, or the enzyme is simultaneously further purified, refolded and buffer-exchanged on a preparative Superdex 200 column by a modified urea reverse-gradient gel filtration | Pseudomonas aeruginosa |
Renatured (Commentary)
| Renatured (Comment) | Organism |
|---|---|
| solubilization of recombinant His-tagged enzyme from strain BL21(DE3) in inclusion bodies by 8 M urea | Pseudomonas aeruginosa |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 3.2 | - |
purified recombinant enzyme from Escherichia coli expression, pH 8.0, 25°C, substrate N-succinyl-Ala-Ala-Ala-4-nitroanilide | Pseudomonas aeruginosa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| N-succinyl-Ala-Ala-Ala-4-nitroanilide + H2O | - |
Pseudomonas aeruginosa | N-succinyl-Ala-Ala-Ala + 4-nitroaniline | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 34160, sequence calculation | Pseudomonas aeruginosa |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| elastase | - |
Pseudomonas aeruginosa |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Pseudomonas aeruginosa |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Pseudomonas aeruginosa |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | pseudolysin belongs to the thermolysin-like family of metallopeptidases | Pseudomonas aeruginosa |
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Release 2023.1 (January 2023)
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