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Literature summary for 3.4.24.26 extracted from
- New continuous and specific fluorometric assays for Pseudomonas aeruginosa elastase and LasA protease (2007), Anal. Biochem., 368, 87-94.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.065 | - |
dabsyl-Ala-Ala-Phe-Ala-EDANS | - |
Pseudomonas aeruginosa |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas aeruginosa | - |
strain PA01 | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
development and evaluation of a highly sensitive assay based on internally quenched fluorogenic peptide substrates comprising an edans (5-(2-aminoethylamino)-1-naphthalenesulfonic acid) group at the C-terminus and a dabsyl (4-(dimethylamino)azobenzene-4'-sulfonyl chloride) fluorophore at the N-terminus of the peptide chains, overview | Pseudomonas aeruginosa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dabsyl-Ala-Ala-Phe-Ala-EDANS + H2O | - |
Pseudomonas aeruginosa | ? | - |
? | |
| additional information | no activity with dabsyl-Leu-Gly-Gly-Gly-Ala-edans | Pseudomonas aeruginosa | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| elastase | - |
Pseudomonas aeruginosa |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 618 | - |
dabsyl-Ala-Ala-Phe-Ala-EDANS | - |
Pseudomonas aeruginosa | |
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