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Literature summary for 3.4.24.26 extracted from

  • Morihara, K.
    Pseudolysin and other pathogen endopeptidases of thermolysin family (1995), Methods Enzymol., 248, 242-253.
    View publication on PubMed
Search for more literature for 3.4.24.26

Crystallization (Commentary)

Crystallization (Comment) Organism
-
 Pseudomonas aeruginosa

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline
-
 Pseudomonas aeruginosa
ClCH2CO-N-hydroxyleucine-Ala-Gly-NH2
-
 Pseudomonas aeruginosa
EDTA
-
 Pseudomonas aeruginosa
additional information diisopropyl fluorophosphate; not: ClCH2CO-N-hydroxyleucine-OCH3; tosyl-L-Phe chloromethyl ketone, PCMB Pseudomonas aeruginosa
peptides containing the hydroxamic acid, N-hydroxypeptide and thiol functional groups Pseudomonas aeruginosa
phosphoramidon
-
 Pseudomonas aeruginosa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.11
-
 2-Aminobenzoyl-Ala-Gly-Leu-Ala 4-nitrobenzylamide
-
 Pseudomonas aeruginosa
0.11
-
 2-aminobenzoyl-Ala-Gly-Leu-Ala-4-nitrobenzylamide
-
 Vibrio cholerae serotype O1

Metals/Ions

Metals/Ions Comment Organism Structure
Zinc essential for activity Pseudomonas aeruginosa
Zinc contains zinc Pseudomonas aeruginosa
Zinc contains 1 atom Zn per molecule Pseudomonas aeruginosa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33000
-
 Pseudomonas aeruginosa Pseudomonas aeruginosa

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa
-
-
-
Vibrio cholerae serotype O1
-
 similar enzyme produced by Vibrio cholerae
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pseudomonas aeruginosa

Source Tissue

Source Tissue Comment Organism Textmining
culture medium
-
 Pseudomonas aeruginosa
-
culture medium
-
 Vibrio cholerae serotype O1
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-Aminobenzoyl-Ala-Gly-Leu-Ala 4-nitrobenzylamide + H2O
-
 Pseudomonas aeruginosa 2-Aminobenzoyl-Ala-Gly + Leu-Ala 4-nitrobenzylamide
-
 ?
2-aminobenzoyl-Ala-Gly-Leu-Ala-4-nitrobenzylamide + H2O
-
 Vibrio cholerae serotype O1 2-aminobenzoyl-Ala-Gly + Leu-Ala-4-nitrobenzylamide
-
 ?
casein + H2O
-
 Pseudomonas aeruginosa ?
-
 ?
Denatured casein + H2O
-
 Pseudomonas aeruginosa ?
-
 ?
Denatured fibrin + H2O
-
 Pseudomonas aeruginosa ?
-
 ?
Denatured hemoglobin + H2O
-
 Pseudomonas aeruginosa ?
-
 ?
Denatured ovalbumin + H2O
-
 Pseudomonas aeruginosa ?
-
 ?
Elastin + H2O
-
 Pseudomonas aeruginosa ?
-
 ?
additional information with synthetic substrates of the general structure benzyloxycarbonyl-Phe-Xaa-Ala, the Phe-Xaa bond is cleaved, the decreasing order of preference for Xaa is as follows: Phe, Leu, Tyr, Val, Ile Pseudomonas aeruginosa ?
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
 pH 7.0, 10 min, in presence of Ca2+ stable Pseudomonas aeruginosa

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
100
-
 2-Aminobenzoyl-Ala-Gly-Leu-Ala 4-nitrobenzylamide
-
 Pseudomonas aeruginosa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 8 casein, elastin Pseudomonas aeruginosa

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 10 4°C, 16 h, in presence of Ca2+ stable Pseudomonas aeruginosa