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Literature summary for 3.4.24.25 extracted from

  • Xie, B.B.; Bian, F.; Chen, X.L.; He, H.L.; Guo, J.; Gao, X.; Zeng, Y.X.; Chen, B.; Zhou, B.C.; Zhang, Y.Z.
    Cold adaptation of zinc metalloproteases in the thermolysin family from deep sea and arctic sea ice bacteria revealed by catalytic and structural properties and molecular dynamics: new insights into relationship between conformational flexibility and hydr (2009), J. Biol. Chem., 284, 9257-9269.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.24.25

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Pseudoalteromonas sp. SM495
expressed in Escherichia coli BL21(DE3) cells Pseudoalteromonas sp. SM9913

Organism

Organism UniProt Comment Textmining
Pseudoalteromonas sp. SM495 B6E623
-
-
Pseudoalteromonas sp. SM9913 A1DRD5
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation and DEAE-Sepharose column chromatography Pseudoalteromonas sp. SM495
ammonium sulfate precipitation and DEAE-Sepharose column chromatography Pseudoalteromonas sp. SM9913

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-(2-furylacryloyl)glycyl-L-leucine + H2O
-
 Pseudoalteromonas sp. SM495 ?
-
 ?
3-(2-furylacryloyl)glycyl-L-leucine + H2O
-
 Pseudoalteromonas sp. SM9913 ?
-
 ?
casein + H2O
-
 Pseudoalteromonas sp. SM495 ?
-
 ?
casein + H2O
-
 Pseudoalteromonas sp. SM9913 ?
-
 ?

Synonyms

Synonyms Comment Organism
E495
-
 Pseudoalteromonas sp. SM495
MCP-02
-
 Pseudoalteromonas sp. SM9913

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
57
-
 using casein as substrate, at pH 7.5 Pseudoalteromonas sp. SM495
57
-
 using casein as substrate, at pH 8.0 Pseudoalteromonas sp. SM9913

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
40 70
-
 Pseudoalteromonas sp. SM495
40 70
-
 Pseudoalteromonas sp. SM9913

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55 64 the melting temperature is at 64°C, the half-life at 55°C is 82 min, the half-life at 60°C is 20 min Pseudoalteromonas sp. SM495
55 64 the melting temperature is at 64°C, the half-life at 55°C is 90 min, the half-life at 60°C is 23 min Pseudoalteromonas sp. SM9913

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 using casein as substrate, at 60°C Pseudoalteromonas sp. SM495
8
-
 using casein as substrate, at 60°C Pseudoalteromonas sp. SM9913

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.32
-
 3-(2-furylacryloyl)glycyl-L-leucine at 25°C, at pH 8.0 Pseudoalteromonas sp. SM9913
2.55
-
 3-(2-furylacryloyl)glycyl-L-leucine at 25°C, at pH 7,5 Pseudoalteromonas sp. SM495