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Literature summary for 3.4.24.25 extracted from
- Aeromonas neutral protease (1976), Methods Enzymol., 45, 404-415.
General Stability
| General Stability | Organism |
|---|---|
| Lyophilization and reconstitution results in approximately 20% loss of activity | Vibrio proteolyticus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 3.5 | - |
Furylacryloyl-Gly-Leu-NH2 | - |
Vibrio proteolyticus |  |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Calcium | contains 1.7 mol of calcium per mol of enzyme, after 48 h of dialysis, not essential for activity, Ca2+ likely plays a structural role | Vibrio proteolyticus | |
| Zinc | essential for activity | Vibrio proteolyticus | |
| Zinc | enzyme contains 1 mol of zinc per mol of enzyme | Vibrio proteolyticus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 34800 | - |
Aeromonas proteolytica, sedimentation equilibrium, sedimentation velocity-diffusion experiments | Vibrio proteolyticus |
| 35000 | - |
1 * 35000 (about), Aeromonas proteolytica, sedimentation equilibrium in presence of guanidine-HCl | Vibrio proteolyticus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Vibrio proteolyticus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Vibrio proteolyticus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| culture filtrate | - |
Vibrio proteolyticus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Vibrio proteolyticus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, Tricine or Tris buffer, stable for several years | Vibrio proteolyticus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| casein + H2O | - |
Vibrio proteolyticus | ? | - |
? | |
| furylacryloyl-Gly-Leu-NH2 + H2O | - |
Vibrio proteolyticus | furylacryloyl-Gly + Leu-NH2 | - |
? | |
| Gelatin + H2O | - |
Vibrio proteolyticus | ? | - |
? | |
| additional information | preference for bonds of the type X-Phe, X-Leu, X-Tyr to which a hydrophobic residue contributes the amino group, and whereas it is essential that the NH2-terminal position of dipeptide substrates be blocked, the enzyme hydrolyzes substrates with free carboxyl groups | Vibrio proteolyticus | ? | - |
? | |
| Urea-denatured hemoglobin + H2O | - |
Vibrio proteolyticus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 35000 (about), Aeromonas proteolytica, sedimentation equilibrium in presence of guanidine-HCl | Vibrio proteolyticus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
or 37°C, assay at | Vibrio proteolyticus |
| 37 | - |
or 25°C, assay at | Vibrio proteolyticus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Vibrio proteolyticus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 11 | 10°C, stable for more than 1 week | Vibrio proteolyticus |
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Release 2023.1 (January 2023)
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