Literature summary for 3.4.24.24 extracted from

Wallon, U.M.; Overall, C.M.
The hemopexin-like domain (C domain) of human gelatinase A (matrix metalloproteinase-2) requires Ca2+ for fibronectin and heparin binding. Binding properties of recombinant gelatinase A C domain to extracellular matrix and basement membrane components (1997), J. Biol. Chem., 272, 7473-7481.

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Cloned(Commentary)

Cloned (Comment)	Organism
cDNA, recombinant C domain expressed in Escherichia coli	Homo sapiens

Inhibitors

Inhibitors	Comment	Organism	Structure
additional information	dithiothreitol does not interfere with heparin binding	Homo sapiens
NaCl	binding to heparin and fibronectin can be disrupted by 0.3 M NaCl	Homo sapiens

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Ca2+	hemopexin-like domain requires Ca2+ for fibronectin and heparin binding	Homo sapiens
Zn2+	structural zinc ion	Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
25920	-	recombinant C domain, elektrospray mass spectrometry	Homo sapiens
26500	-	recombinant C domain, SDS-PAGE	Homo sapiens
72000	-	-	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	human	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant C domain	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
Fibronectin + H2O	-	Homo sapiens	?	-	?
additional information	laminin,native or denatured type I collagen, native or denatured types II, IV, V, and X collagen or the NC1 domain of type VII collagen, elastin,SPARC, tenascin and MatrigelTM are not bound by the recombinant C domain	Homo sapiens	?	-	?

Synonyms

Synonyms	Comment	Organism
72-kDa Gelatinase	-	Homo sapiens
gelatinase A	-	Homo sapiens
matrix metalloproteinase-2	-	Homo sapiens

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Release 2023.1 (January 2023)