Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | the enzyme contains an auto-inhibitory peptide | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | the enzyme is secreted | Homo sapiens | - |
- |
plasma membrane | charge-triggered membrane insertion of matrix metalloproteinase-7. Binding to cholesterol sulfate partially embeds the protease in the bilayer, restricts its diffusion, and tips the active site away from the bilayer. Its insertion of hydrophobic residues organizes the lipids, pushing the head groups and sterol sulfate outward towards the enzyme's positive charge on the periphery of the enlarged interface. Fluorescence probing demonstrates a similar mode of binding to plasma membranes and internalized vesicles of colon cancer cells. Binding of bilayered micelles induces allosteric activation and conformational change in the auto-inhibitory peptide and the adjacent scissile site, illustrating a potential intermediate in the activation of the zymogen. Cholesterol sulfate reorients and inserts proMMP-7 into bicelles. Potential interactions of the II-III loop and interdomain linker with bilayer, NMR structure determination and analysis, overview | Homo sapiens | 5886 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | a zinc-dependent metalloproteinase | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
heparin-binding epidermal growth factor precursor + H2O | Homo sapiens | - |
heparin-binding epidermal growth factor + HB-EGF pro-peptide | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P09237 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | binding of bilayered micelles induces allosteric activation and conformational change in the auto-inhibitory peptide and the adjacent scissile site, illustrating a potential intermediate in the activation of the zymogen | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
Colo-205 cell | - |
Homo sapiens | - |
colonic adenocarcinoma cell | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
heparin-binding epidermal growth factor precursor + H2O | - |
Homo sapiens | heparin-binding epidermal growth factor + HB-EGF pro-peptide | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Matrix metalloproteinase-7 | - |
Homo sapiens |
MMP-7 | - |
Homo sapiens |
General Information | Comment | Organism |
---|---|---|
additional information | determination of NMR structures of the proMMP-7 zymogen free in solution and bound to membrane mimics, as well as by probing with a membrane-responsive fluor. Binding of plasma membranes and internalization, and bicelle-induced removal of auto-inhibitory conformation, overview | Homo sapiens |
physiological function | matrix metalloproteinase-7 (MMP-7) sheds signaling proteins from cell surfaces to activate bacterial killing, wound healing, and tumorigenesis, mechanism targeting soluble MMP-7 to membranes. MMP-7 regulates the remodeling of female reproductive organs by proteolytic activation of heparin-binding epidermal growth factor precursor (pro-HB-EGF) to activate its ErbB4 receptor in uterine and mammary epithelia and tumor cells | Homo sapiens |