Literature summary for 3.4.24.23 extracted from

Ou, L.; Ma, J.; Zheng, X.; Chen, X.; Li, G.; Wu, H.
The expression and refolding of isotopically labeled recombinant matrilysin for NMR studies (2006), Protein Expr. Purif., 47, 367-373.

Search for more literature for 3.4.24.23

Cloned(Commentary)

Cloned (Comment)	Organism
DNA and amino acid sequence determination, overexpression in Escherichia coli strain BL21(DE3) in inclusion bodies	Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
additional information	Homo sapiens	MMP7 plays an essential role in cancer, innate immunity, and in inflammatory disorders	?	-	?

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	P09237	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant enzyme from Escherichia coli strain BL21(DE3) inclusion bodies, after solubilization and refolding, by gel filtration	Homo sapiens

Renatured (Commentary)

Renatured (Comment)	Organism
recombinant enzyme from Escherichia coli strain BL21(DE3) inclusion bodies through solubilization by 8 M urea, followed by anion exchange chromatography and dilution to 6 M urea, refolding in 6 M urea, 20 mM Tris-HCl, 10 mM CaCl2, and 100 mM NaCl, pH 7.5, after refolding dialysis in the same buffer with 10 mM ZnCl2, ultrafiltration, overview	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	MMP7 plays an essential role in cancer, innate immunity, and in inflammatory disorders	Homo sapiens	?	-	?

Subunits

Subunits	Comment	Organism
More	MMP7 structure anaylsis by dynamic light scattering, MALDI-TOF mass spectrometry, and NMR	Homo sapiens

Synonyms

Synonyms	Comment	Organism
MMP7	-	Homo sapiens

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)