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Literature summary for 3.4.24.23 extracted from
- Site-directed mutagenesis of the active site glutamate in human matrilysin: Investigation of its role in catalysis (1997), Biochemistry, 36, 16019-16024.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| wild-type and mutant matrylysin cDNA in plasmid pUN121 expressed in Escherichia coli BL21(DE3) | Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| E198A | site-directed mutagenesis | Homo sapiens |
| E198C | site-directed mutagenesis | Homo sapiens |
| E198D | site-directed mutagenesis | Homo sapiens |
| E198Q | site-directed mutagenesis | Homo sapiens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0079 | - |
dansyl-PLALWAR | pH 7.5, 25°C, mutant E198A | Homo sapiens |  |
| 0.018 | - |
dansyl-PLALWAR | pH 7.5, 25°C, mutant E198C and E198Q | Homo sapiens | |
| 0.021 | - |
dansyl-PLALWAR | pH 7.5, 25°C, mutant E198D | Homo sapiens | |
| 0.024 | - |
dansyl-PLALWAR | pH 7.5, 25°C, wild-type | Homo sapiens | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Zn2+ | zinc protease | Homo sapiens | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
human | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dansyl-PLALWAR + H2O | synthetic fluorescent peptide | Homo sapiens | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| MMP-7 | - |
Homo sapiens |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 4 | - |
purified mutants are stable at | Homo sapiens |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00051 | - |
dansyl-PLALWAR | pH 7.5, 25°C, mutant E198A | Homo sapiens | |
| 0.0036 | - |
dansyl-PLALWAR | pH 7.5, 25°C, mutant E198Q | Homo sapiens | |
| 0.014 | - |
dansyl-PLALWAR | pH 7.5, 25°C, mutant E198C | Homo sapiens | |
| 0.61 | - |
dansyl-PLALWAR | pH 7.5, 25°C, mutant E198D | Homo sapiens | |
| 2.7 | - |
dansyl-PLALWAR | pH 7.5, 25°C, wild-type | Homo sapiens | |
| 6.08 | - |
dansyl-PLALWAR | pH 7.5, 25°C, mutant E198D | Homo sapiens | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Homo sapiens |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | 9.5 | - |
Homo sapiens |
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