Cloned (Comment) | Organism |
---|---|
- |
Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | - |
Homo sapiens | |
DTT | - |
Homo sapiens | |
EDTA | - |
Homo sapiens | |
EGTA | - |
Homo sapiens | |
Specific tissue inhibitor of metalloproteinases | TIMP-1 | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.023 | - |
(7-Methoxycoumarin-4-yl)acetyl-Arg-Pro-Lys-Pro-Val-Glu-norvaline-Trp-Arg-Lys(2,4-dinitrophenyl)-NH2 | - |
Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zinc | zinc metalloenzyme | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
additional information | - |
synthesized as preproenzyme of 476 amino acids and secreted from cells as the proenzyme | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Homo sapiens | participates in activation of procollagenase proMMP-1 | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
additional information | potential glycosylation site at Asn100, but is unlikely to be a glycoprotein as it does not bind concanavalin A | Homo sapiens |
Purification (Comment) | Organism |
---|---|
- |
Homo sapiens |
Storage Stability | Organism |
---|---|
-20°C, prostromelysin 2, 50 mM Tris-HCl, pH 7.5, 0.15 M NaCl, 10 mM CaCl2, 0.02% w/v NaN3, 0.05% Brij 35, stable for 4 weeks | Homo sapiens |
4°C, prostromelysin 2, 50 mM Tris-HCl, pH 7.5, 0.15 M NaCl, 10 mM CaCl2, 0.02% w/v NaN3, 0.05% Brij 35, stable for 2 weeks | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(7-Methoxycoumarin-4-yl)acetyl-Arg-Pro-Lys-Pro-Val-Glu-norvaline-Trp-Arg-Lys(2,4-dinitrophenyl)-NH2 + H2O | - |
Homo sapiens | (7-Methoxycoumarin-4-yl)acetyl-Arg-Pro-Lys-Pro-Val-Glu + norvaline-Trp-Arg-Lys(2,4-dinitrophenyl)-NH2 | - |
? | |
Aggrecan core protein + H2O | - |
Homo sapiens | ? | - |
? | |
Azocoll + H2O | - |
Homo sapiens | ? | - |
? | |
Cartilage link protein + H2O | cleaved at His16-Ile17 bond | Homo sapiens | ? | - |
? | |
Collagen + H2O | type IV | Homo sapiens | ? | - |
? | |
Elastin + H2O | - |
Homo sapiens | ? | - |
? | |
Fibronectin + H2O | negligible | Homo sapiens | ? | - |
? | |
Gelatin + H2O | - |
Homo sapiens | ? | - |
? | |
additional information | participates in activation of procollagenase proMMP-1 | Homo sapiens | ? | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.8 | - |
(7-Methoxycoumarin-4-yl)acetyl-Arg-Pro-Lys-Pro-Val-Glu-norvaline-Trp-Arg-Lys(2,4-dinitrophenyl)-NH2 | - |
Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
activity against aggrecan at pH 5.5 is double that at pH 6.0 | Homo sapiens |
7.5 | 8 | azocoll | Homo sapiens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 8 | 6.0: 25% of activity maximum, 7.5-8.0: activity maximum, azocoll | Homo sapiens |