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Literature summary for 3.4.24.21 extracted from
- Purification and cloning of carp nephrosin, a secreted zinc endopeptidase of the astacin family (1997), J. Biol. Chem., 272, 13772-13778.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Cyprinus carpio |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1,10-phenanthroline | - |
Cyprinus carpio |  |
| captopril | - |
Cyprinus carpio | |
| Diethyldithiocarbamic acid sodium salt | - |
Cyprinus carpio | |
| EDTA | - |
Cyprinus carpio | |
| additional information | little or no inhibition by pestatin, PMSF, and iodoacetate | Cyprinus carpio |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| K+ | secretion of nephrosin can be stimulated by high concentrations of extracellular potassium | Cyprinus carpio | |
| Zn2+ | zinc endopeptidase | Cyprinus carpio | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 23000 | - |
SDS-PAGE | Cyprinus carpio |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cyprinus carpio | O42326 | common carp | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Cyprinus carpio |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
specific activity 18.16 units/mg, one unit of activity is the amount of nephrosin causing the same proteolytic activity as 1 unit of chymotrypsin in the RCM-BSA zymographic assay | Cyprinus carpio |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| bradykinin 1-7 RPPGFSP + H2O | partial digestion | Cyprinus carpio | ? | - |
? | |
| Des-Arg-bradykinin PPGFSPFR + H2O | complete digestion | Cyprinus carpio | ? | - |
? | |
| Lys-bradykinin KRPPGFSPFR + H2O | complete digestion | Cyprinus carpio | ? | - |
? | |
| additional information | tosyl-GPK-NA, Suc-AAPF-NA, Suc-AAPL-NA, Cbz-GGL-NA, bradykinin 1-5, and bradykinin 1-6 are no substrates | Cyprinus carpio | ? | - |
? | |
| PRPKPQQFFGLM-NH2 + H2O | complete digestion | Cyprinus carpio | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| carp nephrosin | - |
Cyprinus carpio |
| nephrosin | - |
Cyprinus carpio |
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