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Literature summary for 3.4.24.21 extracted from
- Fluorescent oligopeptide substrates for kinetic characterization of the specificity of Astacus protease (1990), Biochemistry, 29, 10418-10425.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Dansyl-Gly-Lys-Arg | dansyl-Leu-Lys-Arg-Ala-Pro-Trp-Val as substrate | Astacus astacus |  |
| Dansyl-Leu-Lys-Arg | dansyl-Leu-Lys-Arg-Ala-Pro-Trp-Val as substrate | Astacus astacus | |
| additional information | no inhibition by dansyl-Ala-Ala-Arg, Ala-Pro-Leu-Val | Astacus astacus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.015 | - |
Dansyl-Gly-Lys-Arg-Ala-Pro-Trp-Val | - |
Astacus astacus | |
| 0.017 | - |
Dansyl-Gly-Lys-Arg-Ala-Pro-Leu-Val | - |
Astacus astacus | |
| 0.23 | - |
Dansyl-His-His-Leu-Lys-Arg-Ala-Pro-Trp-Val | dansyl-Gly-Arg-Arg-Ala-Ser-Leu-Gly | Astacus astacus | |
| 0.25 | - |
Dansyl-Val-Lys-Arg-Ala-Pro-Trp-Val | dansyl-Leu-Lys-Arg-Ala-Pro-Leu-Val | Astacus astacus | |
| 0.27 | - |
Dansyl-Gly-Lys-Tyr-Ala-Pro-Trp-Val | - |
Astacus astacus | |
| 0.28 | - |
Dansyl-Leu-Lys-Tyr-Ala-Pro-Trp-Val | - |
Astacus astacus | |
| 0.29 | - |
Dansyl-Leu-Lys-Lys-Ala-Pro-Trp-Val | - |
Astacus astacus | |
| 0.33 | - |
Dansyl-Leu-Lys-Arg-Ala-Pro-Trp-Val | - |
Astacus astacus | |
| 0.37 | - |
Dansyl-Pro-Lys-Arg-Ala-Pro-Trp-Val | - |
Astacus astacus | |
| 0.39 | - |
Dansyl-Gly-Arg-Arg-Ala-Pro-Leu-Gly | - |
Astacus astacus | |
| 0.4 | - |
Dansyl-Gly-Lys-Asn-Ala-Pro-Leu-Val | HPLC analysis | Astacus astacus | |
| 0.48 | - |
Dansyl-Ala-Ala-Arg-Ala-Pro-Leu-Val | - |
Astacus astacus | |
| 0.52 | - |
Dansyl-Gly-Lys-Asn-Ala-Pro-Leu-Val | analyzed by tryptophan fluorescence changes between enzyme-substrate complex and enzyme plus product | Astacus astacus | |
| 0.53 | - |
Dansyl-Gly-Pro-Arg-Ala-Pro-Leu-Val | - |
Astacus astacus | |
| 0.58 | - |
Dansyl-Leu-Arg-Arg-Ala-Pro-Leu-Gly | - |
Astacus astacus | |
| 0.67 | - |
Dansyl-Leu-Arg-Arg-Ala-Ser-Leu-Gly | - |
Astacus astacus | |
| 0.867 | 0.87 | 2-Aminobenzoyl-Arg-Pro-Gly-Leu-Ser-Pro-(4-nitro)Phe-Arg | dansyl-Leu-Lys-Arg-Ala-Pro-Leu | Astacus astacus | |
| 1 | - |
Dansyl-Leu-Lys-Asn-Ala-Pro-Leu-Val | - |
Astacus astacus | |
| 2 | - |
Dansyl-Lys-Arg-Ala-Pro-Leu | - |
Astacus astacus | |
| 5 | - |
Dansyl-Gly-Gly-Arg-Ala-Pro-Trp-Val | - |
Astacus astacus | |
| 56 | - |
Dansyl-Arg-Ala-Pro-Leu | - |
Astacus astacus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| soluble | - |
Astacus astacus | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Zn2+ | requirement | Astacus astacus | |
| Zn2+ | zinc metalloenzyme | Astacus astacus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Astacus astacus | - |
European freshwater crayfish | - |
| Astacus astacus | - |
i.e. Astacus fluviatilis | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| digestive juice | - |
Astacus astacus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-aminobenzoyl-Arg-Pro-Gly-Leu-Ser-Pro-(4-nitro)Phe-Arg + H2O | - |
Astacus astacus | 2-aminobenzoyl-Arg-Pro-Gly-Leu + Ser-Pro-(4-nitro)Phe-Arg | - |
? | |
| Dansyl-Ala-Ala-Arg-Ala-Pro-Leu-Val + H2O | cleavage site: Arg-Ala | Astacus astacus | Dansyl-Ala-Ala-Arg + Ala-Pro-Leu-Val | - |
? | |
| Dansyl-Arg-Ala-Pro-Leu + H2O | cleavage site: Arg-Ala | Astacus astacus | Dansyl-Arg + Ala-Pro-Leu | - |
? | |
| Dansyl-Gly-Arg-Arg-Ala-Pro-Leu-Gly + H2O | cleavage site: Arg-Ala | Astacus astacus | Dansyl-Gly-Arg-Arg + Ala-Pro-Leu-Gly | - |
? | |
| Dansyl-Gly-Arg-Arg-Ala-Ser-Leu-Gly + H2O | cleavage site: Arg-Ala | Astacus astacus | Dansyl-Gly-Arg-Arg + Ala-Ser-Leu-Gly | - |
? | |
| Dansyl-Gly-Gly-Arg-Ala-Pro-Trp-Val + H2O | cleavage site: Arg-Ala | Astacus astacus | Dansyl-Gly-Gly-Arg + Ala-Pro-Trp-Val | - |
? | |
| Dansyl-Gly-Lys-Arg-Ala-Pro-Leu-Val + H2O | cleavage site: Arg-Ala | Astacus astacus | Dansyl-Gly-Lys-Arg + Ala-Pro-Leu-Val | - |
? | |
| Dansyl-Gly-Lys-Arg-Ala-Pro-Trp-Val + H2O | cleavage site: Arg-Ala | Astacus astacus | Dansyl-Gly-Lys-Arg + Ala-Pro-Trp-Val | - |
? | |
| Dansyl-Gly-Lys-Asn-Ala-Pro-Leu-Val + H2O | cleavage site: Asn-Ala | Astacus astacus | Dansyl-Gly-Lys-Asn + Ala-Pro-Leu-Val | - |
? | |
| Dansyl-Gly-Lys-Tyr-Ala-Pro-Trp-Val + H2O | cleavage site: Tyr-Ala | Astacus astacus | Dansyl-Gly-Lys-Tyr + Ala-Pro-Trp-Val | - |
? | |
| Dansyl-Gly-Pro-Arg-Ala-Pro-Leu-Val + H2O | cleavage site: Arg-Ala | Astacus astacus | Dansyl-Gly-Pro-Arg + Ala-Pro-Leu-Val | - |
? | |
| Dansyl-His-His-Leu-Lys-Arg-Ala-Pro-Trp-Val + H2O | cleavage site: Arg-Ala | Astacus astacus | Dansyl-His-His-Leu-Lys-Arg + Ala-Pro-Trp-Val | - |
? | |
| Dansyl-Leu-Arg-Arg-Ala-Pro-Leu-Gly + H2O | cleavage site: Arg-Ala | Astacus astacus | Dansyl-Leu-Arg-Arg + Ala-Pro-Leu-Gly | - |
? | |
| Dansyl-Leu-Arg-Arg-Ala-Ser-Leu-Gly + H2O | cleavage site: Arg-Ala | Astacus astacus | Dansyl-Leu-Arg-Arg + Ala-Ser-Leu-Gly | - |
? | |
| Dansyl-Leu-Lys-Arg-Ala-Pro-Leu + H2O | cleavage site: Arg-Ala | Astacus astacus | Dansyl-Leu-Lys-Arg + Ala-Pro-Leu | - |
? | |
| Dansyl-Leu-Lys-Arg-Ala-Pro-Leu-Val + H2O | cleavage site: Arg-Ala | Astacus astacus | Dansyl-Leu-Lys-Arg + Ala-Pro-Leu-Val | - |
? | |
| Dansyl-Leu-Lys-Arg-Ala-Pro-Trp-Val + H2O | cleavage site: Arg-Ala, poor substrate | Astacus astacus | Dansyl-Leu-Lys-Arg + Ala-Pro-Trp-Val | - |
? | |
| Dansyl-Leu-Lys-Arg-Leu-Pro-Trp-Val + H2O | cleavage site: Arg-Leu, poor substrate | Astacus astacus | Dansyl-Leu-Lys-Arg + Leu-Pro-Trp-Val | - |
? | |
| Dansyl-Leu-Lys-Asn-Ala-Pro-Leu-Val + H2O | cleavage site: Asn-Ala | Astacus astacus | Dansyl-Leu-Lys-Asn + Ala-Pro-Leu-Val | - |
? | |
| Dansyl-Leu-Lys-Lys-Ala-Pro-Trp-Val + H2O | cleavage site: Lys-Ala | Astacus astacus | Dansyl-Leu-Lys-Lys + Ala-Pro-Trp-Val | - |
? | |
| Dansyl-Leu-Lys-Tyr-Ala-Pro-Trp-Val + H2O | cleavage site: Tyr-Ala | Astacus astacus | Dansyl-Leu-Lys-Tyr + Ala-Pro-Trp-Val | - |
? | |
| Dansyl-Lys-Arg-Ala-Pro-Leu + H2O | cleavage site: Arg-Ala | Astacus astacus | Dansyl-Lys-Arg + Ala-Pro-Leu | - |
? | |
| Dansyl-Lys-Glu-Thr-Tyr-Ser-Lys + H2O | poor substrate | Astacus astacus | ? | - |
? | |
| Dansyl-Lys-Glu-Thr-Tyr-Ser-Phe + H2O | poor substrate | Astacus astacus | ? | - |
? | |
| Dansyl-Pro-Gln-Gly-Ile-Ala-Gly-(D)-Arg + H2O | poor substrate | Astacus astacus | ? | - |
? | |
| Dansyl-Pro-Lys-Arg-Ala-Pro-Trp-Val + H2O | cleavage site: Arg-Ala | Astacus astacus | Dansyl-Pro-Lys-Arg + Ala-Pro-Trp-Val | - |
? | |
| Dansyl-Val-Lys-Arg-Ala-Pro-Trp-Val + H2O | cleavage site: Arg-Ala | Astacus astacus | Dansyl-Val-Lys-Arg + Ala-Pro-Trp-Val | - |
? | |
| denatured tubulin + H2O | cleavage pattern | Astacus astacus | hydrolyzed tubulin | - |
? | |
| additional information | No hydrolysis of dansyl-Gly-Gly-Phe-Ala-Gly, dansyl-Phe-Leu-Ala, dansyl-Pro-Ala-Gly, dansyl-Ala-Phe-Phe-Ala, dansyl-Ala-Ala-Phe-Ala, dansyl-Gly-Gly-Phe-Ala, dansyl-Ala-Phe-Leu-Ala, dansyl-Pro-Leu-Gly-Ile-Ala-Gly-(D)-Arg, carbobenzoxy-Gly-Pro-Gly-Gly-Ala-NH2 | Astacus astacus | ? | - |
? | |
| Succinyl-Ala-Ala-Ala 4-nitroanilide + H2O | i.e. STANA, poor substrate | Astacus astacus | Succinyl-Ala-Ala-Ala + p-nitroaniline | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Astacus astacus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.035 | - |
Dansyl-Arg-Ala-Pro-Leu | - |
Astacus astacus | |
| 0.7 | - |
Dansyl-Gly-Arg-Arg-Ala-Ser-Leu-Gly | - |
Astacus astacus | |
| 2.2 | - |
Dansyl-Gly-Lys-Arg-Ala-Pro-Trp-Val | - |
Astacus astacus | |
| 2.3 | - |
Dansyl-Gly-Lys-Arg-Ala-Pro-Leu-Val | - |
Astacus astacus | |
| 2.7 | - |
Dansyl-Leu-Arg-Arg-Ala-Ser-Leu-Gly | - |
Astacus astacus | |
| 20 | - |
Dansyl-Gly-Gly-Arg-Ala-Pro-Trp-Val | - |
Astacus astacus | |
| 20 | - |
Dansyl-Gly-Pro-Arg-Ala-Pro-Leu-Val | - |
Astacus astacus | |
| 34 | - |
Dansyl-Gly-Arg-Arg-Ala-Pro-Leu-Gly | - |
Astacus astacus | |
| 39 | - |
Dansyl-Gly-Lys-Asn-Ala-Pro-Leu-Val | HPLC analysis | Astacus astacus | |
| 40 | - |
Dansyl-Gly-Lys-Tyr-Ala-Pro-Trp-Val | - |
Astacus astacus | |
| 40 | - |
Dansyl-Lys-Arg-Ala-Pro-Leu | - |
Astacus astacus | |
| 43 | - |
Dansyl-Leu-Lys-Arg-Ala-Pro-Leu | - |
Astacus astacus | |
| 56 | - |
Dansyl-Gly-Lys-Asn-Ala-Pro-Leu-Val | analyzed by tryptophan fluorescence changes between enzyme-substrate complex and enzyme plus product | Astacus astacus | |
| 67 | - |
Dansyl-Leu-Lys-Tyr-Ala-Pro-Trp-Val | - |
Astacus astacus | |
| 120 | - |
Dansyl-Leu-Lys-Arg-Ala-Pro-Leu-Val | - |
Astacus astacus | |
| 130 | - |
Dansyl-Leu-Arg-Arg-Ala-Pro-Leu-Gly | - |
Astacus astacus | |
| 180 | - |
Dansyl-Leu-Lys-Asn-Ala-Pro-Leu-Val | - |
Astacus astacus | |
| 190 | - |
Dansyl-Val-Lys-Arg-Ala-Pro-Trp-Val | - |
Astacus astacus | |
| 200 | - |
Dansyl-Ala-Ala-Arg-Ala-Pro-Leu-Val | - |
Astacus astacus | |
| 210 | - |
Dansyl-Leu-Lys-Arg-Ala-Pro-Trp-Val | - |
Astacus astacus | |
| 210 | - |
Dansyl-Leu-Lys-Lys-Ala-Pro-Trp-Val | - |
Astacus astacus | |
| 310 | - |
Dansyl-His-His-Leu-Lys-Arg-Ala-Pro-Trp-Val | - |
Astacus astacus | |
| 380 | - |
Dansyl-Pro-Lys-Arg-Ala-Pro-Trp-Val | - |
Astacus astacus | |
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