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Literature summary for 3.4.24.20 extracted from
- Characterization of a thermostable lysine-specific metalloendopeptidase from the fruiting bodies of a basidiomycete, Grifola frondosa (1995), J. Biochem., 118, 1014-1020.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1,10-phenanthroline | strong, caseinolytic activity | Grifola frondosa |  |
| 2,2'-bipyridine | strong, caseinolytic activity | Grifola frondosa | |
| 2-mercaptoethanol | weak, caseinolytic activity | Grifola frondosa | |
| 6-aminohexanoic acid | caseinolytic activity | Grifola frondosa | |
| agmatine | caseinolytic activity | Grifola frondosa | |
| antipain | weak, caseinolytic activity | Grifola frondosa | |
| Ba2+ | 1 mM, weak, caseinolytic activity | Grifola frondosa | |
| Ca2+ | 1 mM, weak, caseinolytic activity | Grifola frondosa | |
| Co2+ | 0.1 mM, caseinolytic activity | Grifola frondosa | |
| Cu2+ | 1 mM, caseinolytic activity | Grifola frondosa | |
| dithiothreitol | caseinolytic activity; strong | Grifola frondosa | |
| DL-homoarginine | caseinolytic activity | Grifola frondosa | |
| EDTA | fully restorable by Ca2+; fully restorable by Zn2+, Mn2+, Co2+; strong, caseinolytic activity | Grifola frondosa | |
| Hg2+ | strong, caseinolytic activity | Grifola frondosa | |
| L-aminobutyric acid | caseinolytic activity | Grifola frondosa | |
| L-arginine | caseinolytic activity | Grifola frondosa | |
| L-lysine | caseinolytic activity | Grifola frondosa | |
| L-Lysine ethylester | caseinolytic activity | Grifola frondosa | |
| L-ornithine | caseinolytic activity | Grifola frondosa | |
| Mg2+ | 1 mM, weak, caseinolytic activity | Grifola frondosa | |
| additional information | no inhibition (of caseinolytic activity) by chymostatin, iodoacetate, leupeptin; PMSF; zinc acetate, elastatinal, Lys-Lys, 4 M urea | Grifola frondosa | |
| p-chloromercuribenzoate | not | Grifola frondosa | |
| phosphoramidon | weak, caseinolytic activity | Grifola frondosa | |
| tosyl-Lys chloromethyl ketone | not | Grifola frondosa | |
| tosyl-Phe chloromethyl ketone | weak, caseinolytic activity | Grifola frondosa | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Grifola frondosa | 5829 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | activation, zinc-depleted apoenzyme | Grifola frondosa | |
| Co2+ | activation, zinc-depleted apoenzyme | Grifola frondosa | |
| Mn2+ | activation, 0.1 mM, native enzyme and zinc-depleted apoenzyme | Grifola frondosa | |
| Zinc | requirement, 1 atom per molecule of native enzyme | Grifola frondosa | |
| Zinc | Zn2+ activates zinc-depleted apoenzyme | Grifola frondosa | |
| Zinc | atomic absorption spectroscopy | Grifola frondosa | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 20000 | - |
x * 20000, Grifola frondosa, SDS-PAGE | Grifola frondosa |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Grifola frondosa | - |
i.e. Maitake, var. S.F.Gray | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Grifola frondosa |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| fruit body | - |
Grifola frondosa | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Grifola frondosa |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -40°C, pH 7, at least a year | Grifola frondosa |
| 4°C, in various buffers, pH 5-10, about 20% loss of activity within 18 h | Grifola frondosa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| azocasein + H2O | - |
Grifola frondosa | ? | - |
? | |
| Azocoll + H2O | general protease substrate | Grifola frondosa | ? | - |
? | |
| Bovine serum albumin + H2O | - |
Grifola frondosa | ? | - |
? | |
| Congo red elastin + H2O | - |
Grifola frondosa | ? | - |
? | |
| Equine heart cytochrome c + H2O | cleaves-X-Lys bonds | Grifola frondosa | ? | - |
? | |
| additional information | specific cleavage of acyl-Lys bonds (-X-Lys-) in polypeptides | Grifola frondosa | ? | - |
? | |
| protamine sulfate + H2O | - |
Grifola frondosa | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 20000, Grifola frondosa, SDS-PAGE | Grifola frondosa |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Grifola frondosa |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
3 h, about 30% loss of activity at pH 9 | Grifola frondosa |
| 60 | - |
3 h, about 55% loss of activity at pH 9 | Grifola frondosa |
| 70 | - |
and below, 3 h stable at pH 7.2 | Grifola frondosa |
| 70 | - |
3 h, about 90% loss of activity at pH 9 | Grifola frondosa |
| 70 | - |
3 h, about 40% loss of activity with Mn2+ instead of zinc | Grifola frondosa |
| 80 | - |
3 h, 40% loss of activity at pH 7.2, 30 min, about 90% loss of activity with Mn2+ instead of zinc | Grifola frondosa |
| 100 | - |
30 min, inactivation | Grifola frondosa |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
pI: 7.46 | Grifola frondosa |
| 9 | - |
congo red elastin as substrate | Grifola frondosa |
| 9.5 | - |
azocoll or azocasein as substrate | Grifola frondosa |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 10.5 | more than 50% of maximal activity at pH 6 and 10.5 | Grifola frondosa |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 10 | 18 h, about 20% loss of activity at 4°C | Grifola frondosa |
| 7.2 | - |
3 h, stable at 70°C and below, 40% loss of activity at 80°C, inactivation within 30 min at 100°C | Grifola frondosa |
| 9 | - |
3 h, about 30%, 55% and 90% loss of activity at 50°C, 60°C and 70°C, respectively | Grifola frondosa |
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