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Literature summary for 3.4.24.18 extracted from
- Meprin metalloproteases inactivate interleukin 6 (2014), J. Biol. Chem., 289, 7580-7588.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| recombinant expression of C-terminally His6-tagged meprin alpha protein (1-615) in human HEK-293 cells | Mus musculus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| actinonin | - |
Homo sapiens |  |
| actinonin | - |
Mus musculus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| brush border membrane | - |
Mus musculus | 31526 | - |
| brush border membrane | - |
Homo sapiens | 31526 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Zn2+ | required, zinc metalloprotease | Mus musculus | |
| Zn2+ | required, zinc metalloprotease | Homo sapiens | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
| Mus musculus | - |
- |
- |
| Mus musculus C57BL6 | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| proteolytic modification | activation of recombinant zymogen enzyme by trypsin | Mus musculus |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His6-tagged meprin alpha protein (1-615) from HEK-293 cells by metal-chelating affinity chromatography | Mus musculus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| kidney | - |
Mus musculus | - |
| kidney | - |
Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| interleukin-6 + H2O | recombinant human substrate expressed in an eukaryotic cell line, inactivation | Mus musculus | ? | - |
? | |
| interleukin-6 + H2O | recombinant human substrate expressed in eukaryotic B9 cell line, inactivation. Human meprin A cleaves 20% of human interleukin-6 of about 22 kDa to a smaller product of about 21 kDa within 2 h. Meprin A cleaves human interleukin-6 at its C-terminus, fragmentation pattern, overview | Homo sapiens | ? | - |
? | |
| interleukin-6 + H2O | recombinant murine substrate expressed in Escherichia coli, inactivation. The enzyme cleaves 90% of the substrate within 2 h, approximately 50 and 15% of the mIL-6 signal remain after 0.5 and 1 h of incubation with mouse meprin A, fragmentation pattern, overview | Mus musculus | ? | - |
? | |
| interleukin-6 + H2O | recombinant human substrate expressed in an eukaryotic cell line, inactivation | Mus musculus C57BL6 | ? | - |
? | |
| interleukin-6 + H2O | recombinant murine substrate expressed in Escherichia coli, inactivation. The enzyme cleaves 90% of the substrate within 2 h, approximately 50 and 15% of the mIL-6 signal remain after 0.5 and 1 h of incubation with mouse meprin A, fragmentation pattern, overview | Mus musculus C57BL6 | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| meprin alpha | - |
Mus musculus |
| meprin alpha | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Mus musculus |
| 37 | - |
assay at | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Mus musculus |
| 7.5 | - |
assay at | Homo sapiens |
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Release 2023.1 (January 2023)
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