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Literature summary for 3.4.24.18 extracted from

  • Herzog, C.; Haun, R.S.; Ludwig, A.; Shah, S.V.; Kaushal, G.P.
    ADAM10 is the major sheddase responsible for the release of membrane-associated meprin A (2014), J. Biol. Chem., 289, 13308-13322.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
stable expression of HA-tagged alpha-and beta-subunits in HEK-293 cells and secretion to the culture medium Homo sapiens
stable expression of HA-tagged alpha-subunit in HEK-293 cells and secretion to the culture medium Homo sapiens
stable expression of HA-tagged beta-subunit in HEK-293 cells and secretion to the culture medium Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
TAPI-1 a hydroxamate inhibitor; a hydroxamate inhibitor Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane ADAM10 is the major sheddase responsible for the release of membrane-associated meprin A Homo sapiens 16020
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Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-
Homo sapiens Q16819 subunit alpha
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Homo sapiens Q16820 subunit beta
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Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification ADAM10 is the major sheddase responsible for the release of membrane-associated meprin A Homo sapiens
proteolytic modification ADAM10 is the major sheddase responsible for the release of membrane-associated meprin A. ADAM10 is the protease responsible for the shedding of meprin beta from the meprin beta and meprin alphabeta transfectants, determination by inhibiotr experiments. Both constitutive as well as PMA- and ionomycin-induced shedding of meprin A in the primary renal tubular epithelial cells is markedly prevented by the hydroxamate inhibitor TAPI-1 and the ADAM10-specific inhibitor GI254023X Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
recombinant extracellular HA-tagged alpha-subunit from HEK-293 cell culture medium by ultrafiltration Homo sapiens
recombinant extracellular HA-tagged beta-subunit from HEK-293 cell culture medium by ultrafiltration Homo sapiens
recombinant extracellular HA-tagged enzyme dimer from HEK-293 cell culture medium by ultrafiltration Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
kidney
-
Homo sapiens
-
renal proximal tubule meprin A, composed of alpha and beta subunits, is the major form of meprin present in the apical membranes of renal proximal tubules Homo sapiens
-

Subunits

Subunits Comment Organism
heterodimer alphabeta Homo sapiens