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Literature summary for 3.4.24.18 extracted from

  • Madoux, F.; Tredup, C.; Spicer, T.P.; Scampavia, L.; Chase, P.S.; Hodder, P.S.; Fields, G.B.; Becker-Pauly, C.; Minond, D.
    Development of high throughput screening assays and pilot screen for inhibitors of metalloproteases meprin alpha and beta (2014), Biopolymers, 102, 396-406.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.24.18

Cloned(Commentary)

Cloned (Comment) Organism
recombinant expression of Strep-tagged enzyme in Spodoptera frugiperda Sf9 insect cells Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
3-[(E)-[4-formyl-5-hydroxy-6-methyl-3-[(phosphonooxy)methyl]pyridin-2-yl]diazenyl]-7-nitronaphthalene-1,5-disulfonic acid competitive inhibition Homo sapiens
additional information inhibitor screening, overview. Poor inhibition by NF449, a suramin analogue Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ a zinc metalloproteinase Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification recombinant enzyme activation by trypsin Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
recombinant Strep-tagged enzyme from Spodoptera frugiperda Sf9 insect cells by affinity chromatography Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(7-methoxycoumarin-4-yl)acetyl-YVADAPK-(K-epsilon-DNP)-NH2 + H2O
-
 Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
meprin alpha
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Homo sapiens

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.067
-
 pH 7.5, 25°C Homo sapiens 3-[(E)-[4-formyl-5-hydroxy-6-methyl-3-[(phosphonooxy)methyl]pyridin-2-yl]diazenyl]-7-nitronaphthalene-1,5-disulfonic acid