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Literature summary for 3.4.24.18 extracted from
- An old enzyme with a new function: purification and characterization of a distinct matrix-degrading metalloproteinase in rat kidney cortex and its identification as meprin (1994), J. Cell Biol., 126, 1319-1327.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no activation by organomercurials or trypsin treatment | Rattus norvegicus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1,10-phenanthroline | - |
Rattus norvegicus |  |
| DTT | strong | Rattus norvegicus | |
| EDTA | - |
Rattus norvegicus | |
| additional information | leupeptin; L-trans-epoxysuccinyl-leucylamido-(4-guanidino)-butane (i.e. E-64); NEM, tissue inhibitor of metalloproteinases; no inhibition by phosphoramidon; PMSF, pepstatin; soybean trypsin inhibitor | Rattus norvegicus |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | - |
Rattus norvegicus | 16020 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 140000 | 180000 | rat, SDS-PAGE, non-reducing conditions | Rattus norvegicus |
| 350000 | - |
rat, gel filtration | Rattus norvegicus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | - |
Rattus norvegicus |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| kidney | cortex | Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
0.096 mg gelatin/min/mg | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Collagen type IV + H2O | - |
Rattus norvegicus | Hydrolyzed collagen type IV | - |
? | |
| Fibronectin + H2O | - |
Rattus norvegicus | Hydrolyzed fibronectin | - |
? | |
| Gelatin + H2O | - |
Rattus norvegicus | Hydrolyzed gelatin | - |
? | |
| laminin + H2O | - |
Rattus norvegicus | fragments of laminin | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | SDS-PAGE | Rattus norvegicus |
| tetramer | under reducing conditions | Rattus norvegicus |
| tetramer | rat | Rattus norvegicus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Rattus norvegicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 8 | gelatin as substrate | Rattus norvegicus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 9 | rapid decrease of activity below 5 and above 9 | Rattus norvegicus |
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Release 2023.1 (January 2023)
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