Cloned (Comment) | Organism |
---|---|
expressed in HEK293 cells | Mus musculus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Mus musculus | 16020 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Mus musculus | membrane-bound variant of neurolysin functions as the non-angiotensin Type 1 (non-AT1), non-AT2 Angiotensin binding site | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
a two-step purification procedure based on the molecular size and isoelectric point of the photoradiolabeled binding protein is conducted. Purified samples are subjected to two-dimensional gel electrophoresis followed by mass spectrometry identification of proteins in the two-dimensional gel sections containing radioactivity. LC-MS/MS analysis reveal protein candidates, of which the most abundant are immunoprecipitated after photoradiolabeling. Immunoprecipitation studies indicate that the angiotensin binding site is the membrane-bound variant of metalloendopeptidase neurolysin | Mus musculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Mus musculus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | membrane-bound variant of neurolysin functions as the non-angiotensin Type 1 (non-AT1), non-AT2 Angiotensin binding site | Mus musculus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
neurolysin | - |
Mus musculus |
General Information | Comment | Organism |
---|---|---|
malfunction | brain membranes of mice lacking neurolysin are nearly devoid of the non-AT1, non-AT2 angiotensin binding site | Mus musculus |