Protein Variants | Comment | Organism |
---|---|---|
Y610L | marked change in substrate specificity with 3fold higher binding efficiency to matrix metalloproteinase MMP-2/9 substrate (7-methoxy-coumarin-4-yl)acetyl-RPKPYA-Nva-WMK(2,4-dinitrophenyl)-NH2 with glutamic acid at the P2' amino acid position | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
6 | - |
(7-methoxy-coumarin-4-yl)acetyl-RPKPVE-Nva-WRK(2,4-dinitrophenyl)-NH2 | mutant Y610L, 37°C | Rattus norvegicus | |
7.7 | - |
(7-methoxy-coumarin-4-yl)acetyl-RPKPYA-Nva-WMK(2,4-dinitrophenyl)-NH2 | wild-type, 37°C | Rattus norvegicus | |
14 | - |
(7-methoxy-coumarin-4-yl)acetyl-RPKPVE-Nva-WRK(2,4-dinitrophenyl)-NH2 | wild-type, 37°C | Rattus norvegicus | |
19 | - |
(7-methoxy-coumarin-4-yl)acetyl-RPKPYA-Nva-WMK(2,4-dinitrophenyl)-NH2 | mutant Y610L, 37°C | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(7-methoxy-coumarin-4-yl)acetyl-RPKPVE-Nva-WRK(2,4-dinitrophenyl)-NH2 + H2O | substrate of matrix metalloproteinases MMP3 | Rattus norvegicus | (7-methoxy-coumarin-4-yl)acetyl-RPKP + YA-Nva-WMK(2,4-dinitrophenyl)-NH2 | cleavage at the peptide bond between proline and tyrosine, withabout 1/10 efficiency in wild-type compared with the cleavage of the MMPs-2/9-specific peptide (7-methoxy-coumarin-4-yl)acetyl-RPKPYA-Nva-WMK(2,4-dinitrophenyl)-NH2 | ? | |
(7-methoxy-coumarin-4-yl)acetyl-RPKPYA-Nva-WMK(2,4-dinitrophenyl)-NH2 + H2O | substrate of matrix metalloproteinases MMP-2/-9 | Rattus norvegicus | (7-methoxy-coumarin-4-yl)acetyl-RPKP + YA-Nva-WMK(2,4-dinitrophenyl)-NH2 | cleavage at the peptide bond between proline and tyrosine | ? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.76 | - |
(7-methoxy-coumarin-4-yl)acetyl-RPKPVE-Nva-WRK(2,4-dinitrophenyl)-NH2 | wild-type, 37°C | Rattus norvegicus | |
1.1 | - |
(7-methoxy-coumarin-4-yl)acetyl-RPKPVE-Nva-WRK(2,4-dinitrophenyl)-NH2 | mutant Y610L, 37°C | Rattus norvegicus | |
1.9 | - |
(7-methoxy-coumarin-4-yl)acetyl-RPKPYA-Nva-WMK(2,4-dinitrophenyl)-NH2 | mutant Y610L, 37°C | Rattus norvegicus | |
2.1 | - |
(7-methoxy-coumarin-4-yl)acetyl-RPKPYA-Nva-WMK(2,4-dinitrophenyl)-NH2 | wild-type, 37°C | Rattus norvegicus |