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Literature summary for 3.4.24.16 extracted from

  • Paschoalin, T.; Carmona, A.K.; Rodrigues, E.G.; Oliveira, V.; Monteiro, H.P.; Juliano, M.A.; Juliano, L.; Travassos, L.R.
    Characterization of thimet oligopeptidase and neurolysin activities in B16F10-Nex2 tumor cells and their involvement in angiogenesis and tumor growth (2007), Mol. Cancer, 6, 44.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) Mus musculus

Inhibitors

Inhibitors Comment Organism Structure
JA-2 almost complete inhibition at 0.003 mM Mus musculus
additional information no inhibition by bestatin, captopril, leupeptin, and E64, poor inhibition by PMSF and Z-Pro-prolinal Mus musculus
o-phenanthroline complete inhibition at 4 mM Mus musculus
Pro-Ile 48% inhibition at 1 mM Mus musculus
S-nitroso-N-acetylpenicillamine inhibition is prevented by DTT Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
bradykinin + H2O Mus musculus
-
?
-
?
additional information Mus musculus the enzyme is involved in angiogenesis and tumor growth, overview ?
-
?
neurotensin + H2O Mus musculus
-
?
-
?

Organism

Organism UniProt Comment Textmining
Mus musculus
-
C57BL/6 mice
-
Mus musculus C57BL/6
-
C57BL/6 mice
-

Source Tissue

Source Tissue Comment Organism Textmining
B16F10-Nex2 cell melanoma cell line Mus musculus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
substrate specificity, and cell proliferation assay, overview Mus musculus
0.0088
-
purified recombinant enzyme, substrate peptide RPPGFSPFRQ Mus musculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Abz-GFSIFR-EDDnp + H2O
-
Mus musculus Abz-GFSI + FR-EDDnp
-
?
Abz-GFSPFR-EDDnp + H2O
-
Mus musculus Abz-GFS + PFR-EDDnp
-
?
Abz-GFSPFRQ-EDDnp + H2O
-
Mus musculus Abz-GFSP + FRQ-EDDnp
-
?
Abz-NKPRRPQ-EDDnp + H2O
-
Mus musculus Abz-NKPR + RPQ-EDDnp
-
?
Abz-RPPGFSPFRQ-EDDnp + H2O
-
Mus musculus Abz-RPPGFS + PFRQ-EDDnp
-
?
bradykinin + H2O
-
Mus musculus ?
-
?
additional information the enzyme is involved in angiogenesis and tumor growth, overview Mus musculus ?
-
?
additional information substrate and cleavage site specificity, overview, no activity with GFPPFRQ Mus musculus ?
-
?
neurotensin + H2O
-
Mus musculus ?
-
?
neurotensin + H2O cleavage of Pro-Tyr bonds Mus musculus ?
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Mus musculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
assay at Mus musculus