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Literature summary for 3.4.24.15 extracted from

  • Kessler, J.H.; Khan, S.; Seifert, U.; Le Gall, S.; Chow, K.M.; Paschen, A.; Bres-Vloemans, S.A.; de Ru, A.; van Montfoort, N.; Franken, K.L.; Benckhuijsen, W.E.; Brooks, J.M.; van Hall, T.; Ray, K.; Mulder, A.; Doxiadis, I.I.; van Swieten, P.F.; Overkleeft, H.S.; Prat, A.; Tomkinson, B.; Neefjes, J.; Klo, K.l.o.e.
    Antigen processing by nardilysin and thimet oligopeptidase generates cytotoxic T cell epitopes (2011), Nat. Immunol., 12, 45-53.
    View publication on PubMed
Search for more literature for 3.4.24.15

Cloned(Commentary)

Cloned (Comment) Organism
expressed in HeLa cells and in Escherichia coli Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ELFSYLIEKVKR + H2O purified TOP efficiently cleaves the nardilysin-dependent 12- and 13-residue precursors PRA(190-201) (ELFSYLIEKVKR) and PRA(190-202) (ELFSYLIEKVKRK) directly after the epitope's C-terminal Lys198 Homo sapiens ELFSYLIEK + VKR
-
 ?
ELFSYLIEKVKRK + H2O purified TOP efficiently cleaves the nardilysin-dependent 12- and 13-residue precursors PRA(190-201) (ELFSYLIEKVKR) and PRA(190-202) (ELFSYLIEKVKRK) directly after the epitope’s C-terminal Lys198 Homo sapiens ELFSYLIEK + VKRK
-
 ?

Synonyms

Synonyms Comment Organism
thimet oligopeptidase
-
 Homo sapiens
TOP
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Homo sapiens

General Information

General Information Comment Organism
malfunction suppression of TOP by RNAi results in much less recognition of K562-A3 cells by anti-ELFSYLIEK CTL indicating an involvement of TOP in the endogenous generation of ELFYSLIEK (CTL epitope) Homo sapiens
physiological function Nardilysin and TOP are required, either together or alone, for the generation of a tumor-specific CTL epitope from PRAME, an immunodominant CTL epitope from Epstein-Barr virus protein EBNA3C, and a clinically important epitope from the melanoma protein MART-1. TOP functions as C-terminal trimming peptidase in antigen processing, and nardilysin contributes to both the C-terminal and N-terminal generation of CTL epitopes Homo sapiens