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Literature summary for 3.4.24.15 extracted from

  • Cleverly, K.; Wu, T.J.
    Is the metalloendopeptidase EC 3.4.24.15 (EP24.15), the enzyme that cleaves luteinizing hormone-releasing hormone (LHRH), an activating enzyme? (2010), Reproduction, 139, 319-330.
    View publication on PubMed
Search for more literature for 3.4.24.15

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm EP24.15 is predominantly located in the cytoplasm and is secreted into the extracellular space Rattus norvegicus 5737
-
extracellular EP24.15 is predominantly located in the cytoplasm and is secreted into the extracellular space Rattus norvegicus
-
-
additional information EP24.15 does not contain a membrane anchoring motif yet it is localised to the extracellular surface of the plasma membrane Rattus norvegicus
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ dependent on Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
75000
-
-
 Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
pyroGlu-His-Trp-Ser-Tyr-Gly-Leu-Arg-Pro-Gly + H2O Rattus norvegicus i.e. luteinizing hormone-releasing hormone or LHRH-I, EP24.15 can convert the decapeptide LHRH-I to the active pentapeptide LHRH-(1–5). EP24.15 has a biomodulating action, forming a peptide product that opposes the action of its parent peptide pyroGlu-His-Trp-Ser-Tyr + Gly-Leu-Arg-Pro-Gly + ? i.e. luteinizing hormone-releasing hormone(1-5) or LHRH-(1-5), the fragment does not bind to LHRH-receptor instead it has antagonistic properties at the N-methyl-D-aspartic acid receptor ?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
phosphoprotein the enzyme activity is dependent upon phosphorylation by protein kinase A on serine residue 644. Phosphorylation of this conserved site reduces enzyme affinity for binding LHRH-I Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
 Rattus norvegicus
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information substrate specificity of EP24.15, overview Rattus norvegicus ?
-
 ?
pyroGlu-His-Trp-Ser-Tyr-Gly-Leu-Arg-Pro-Gly + H2O i.e. luteinizing hormone-releasing hormone or LHRH-I, EP24.15 that cleaves the hormone at the fifth and sixth bond of the decapeptide (Tyr5-Gly6) to form LHRH-(1–5) Rattus norvegicus pyroGlu-His-Trp-Ser-Tyr + Gly-Leu-Arg-Pro-Gly i.e. luteinizing hormone-releasing hormone(1-5) or LHRH-(1-5) ?
pyroGlu-His-Trp-Ser-Tyr-Gly-Leu-Arg-Pro-Gly + H2O i.e. luteinizing hormone-releasing hormone or LHRH-I, EP24.15 can convert the decapeptide LHRH-I to the active pentapeptide LHRH-(1–5). EP24.15 has a biomodulating action, forming a peptide product that opposes the action of its parent peptide Rattus norvegicus pyroGlu-His-Trp-Ser-Tyr + Gly-Leu-Arg-Pro-Gly + ? i.e. luteinizing hormone-releasing hormone(1-5) or LHRH-(1-5), the fragment does not bind to LHRH-receptor instead it has antagonistic properties at the N-methyl-D-aspartic acid receptor ?

Synonyms

Synonyms Comment Organism
EP24.15
-
 Rattus norvegicus
metalloendopeptidase EC 3.4.24.15
-
 Rattus norvegicus
More the enzyme is a Zn-dependent a thermolysin like metalloendopeptidase Rattus norvegicus

General Information

General Information Comment Organism
metabolism EP24.15 is the main enzyme of luteinizing hormone-releasing hormone, i.e. LHRH, metabolism as the prime mediator of LHRH-I degradation in both the brain and periphery Rattus norvegicus
physiological function many roles of EP24.15 in the metabolism of neuropeptides and in cell regulation, overview Rattus norvegicus