General Stability | Organism |
---|---|
Ca2+ stabilizes | Gallus gallus |
Ca2+ stabilizes | Bos taurus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | - |
Bos taurus | |
1,10-phenanthroline | 100% inhibition at 1 mM | Gallus gallus | |
2-mercaptoethanol | - |
Bos taurus | |
2-mercaptoethanol | 78% inhibition at 1 mM | Gallus gallus | |
concanavalin A | - |
Bos taurus | |
concanavalin A | 74% inhibition at 50 micromol/ml | Gallus gallus | |
Cu2+ | - |
Bos taurus | |
Cu2+ | 63% inhibition at 0.1 mM | Gallus gallus | |
EDTA | - |
Bos taurus | |
EDTA | 100% inhibition at 7.5 mM | Gallus gallus | |
Fetal bovine serum | - |
Bos taurus | |
Fetal bovine serum | 56% inhibition at concentration of 2% | Gallus gallus | |
heparan sulfate | - |
Bos taurus | |
heparan sulfate | 50% inhibition at 0.05 micromol/ml | Gallus gallus | |
heparin | 76% inhibition at 0.05 micromol/ml | Bos taurus | |
heparin | 76% inhibition at 0.05 micromol/ml | Gallus gallus | |
NaCl | 40% inhibition at 0.3 M | Gallus gallus | |
Ni2+ | - |
Bos taurus | |
Ni2+ | 42% inhibition at 0.1 mM | Gallus gallus | |
phosphate | - |
Bos taurus | |
phosphate | 100% inhibition at 10 mM sodium phosphate | Gallus gallus | |
Poly-L-aspartate | - |
Bos taurus | |
Poly-L-aspartate | 81% inhibition at 10 micromol/ml | Gallus gallus | |
Poly-L-glutamate | - |
Bos taurus | |
Poly-L-glutamate | 89% inhibition at 10 micromol/ml | Gallus gallus | |
SDS | - |
Bos taurus | |
SDS | 84% inhibition at 50 micromol/ml | Gallus gallus | |
Tris-HCl | 80% inhibition at 0.3 M | Gallus gallus | |
Zn2+ | - |
Bos taurus | |
Zn2+ | 92% inhibition at 0.1 mM | Gallus gallus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000054 | - |
procollagen I | - |
Gallus gallus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ba2+ | increase of activity | Gallus gallus | |
Ba2+ | increase of activity | Bos taurus | |
Ba2+ | 70% as effective as Ca2+ | Gallus gallus | |
Ca2+ | required for maximal activity | Gallus gallus | |
Ca2+ | required for maximal activity | Bos taurus | |
Ca2+ | optimal activity at 5 to 10 mM | Gallus gallus | |
Ca2+ | optimal activity at 5 to 10 mM | Bos taurus | |
Mg2+ | increase of activity | Gallus gallus | |
Mg2+ | increase of activity | Bos taurus | |
Mg2+ | 30% as effective as Ca2+ | Gallus gallus | |
Mn2+ | increase of activity | Gallus gallus | |
Mn2+ | increase of activity | Bos taurus | |
Mn2+ | 70% as effective as Ca2+ | Gallus gallus | |
Sr2+ | increase of activity | Gallus gallus | |
Sr2+ | increase of activity | Bos taurus | |
Sr2+ | 85% as effective as Ca2+ | Gallus gallus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
500000 | - |
gel filtration | Gallus gallus |
500000 | - |
gel filtration | Bos taurus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
procollagen I + H2O | Gallus gallus | - |
? | - |
? | |
procollagen I + H2O | Bos taurus | - |
? | - |
? | |
procollagen II + H2O | Gallus gallus | - |
? | - |
? | |
procollagen II + H2O | Bos taurus | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Gallus gallus | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
side-chain modification | glycoprotein | Gallus gallus |
side-chain modification | glycoprotein | Bos taurus |
Purification (Comment) | Organism |
---|---|
- |
Gallus gallus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
skin | - |
Bos taurus | - |
tendon | - |
Gallus gallus | - |
tendon | - |
Bos taurus | - |
Storage Stability | Organism |
---|---|
4 to -20°C, 0.05 M Tris-HCl, 0.75 M NaCl, 0.02% Brij 35, 0.01% NaN3, pH 7.5, 1-2 months, 90% activity recovered | Gallus gallus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
procollagen I + H2O | not procollagen III | Gallus gallus | pCCollagen I + oligopeptide | partially processed procollagen containing only the C-propeptides | ? | |
procollagen I + H2O | not procollagen III | Bos taurus | pCCollagen I + oligopeptide | partially processed procollagen containing only the C-propeptides | ? | |
procollagen I + H2O | - |
Gallus gallus | ? | - |
? | |
procollagen I + H2O | - |
Bos taurus | ? | - |
? | |
procollagen II + H2O | - |
Gallus gallus | pCCollagen II + oligopeptide | partially processed procollagen containing only the C-propeptides | ? | |
procollagen II + H2O | - |
Bos taurus | pCCollagen II + oligopeptide | partially processed procollagen containing only the C-propeptides | ? | |
procollagen II + H2O | - |
Gallus gallus | ? | - |
? | |
procollagen II + H2O | - |
Bos taurus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
oligomer | SDS-PAGE | Gallus gallus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0967 | - |
procollagen I | - |
Gallus gallus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.9 | 8.3 | procollagen I | Gallus gallus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
inactive below, procollagen I as substrate | Gallus gallus |
5.5 | - |
inactive below, procollagen I as substrate | Bos taurus |
6.8 | 9.5 | procollagen I as substrate, 20% of optimum activity at pH 6.8 and pH 9.5 | Gallus gallus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5.5 | 10 | type I/II procollagen N-proteinase | Gallus gallus |