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Literature summary for 3.4.24.11 extracted from
- Heterologous expression and characterization of recombinant Lactococcus lactis neutral endopeptidase (neprilysin) (1996), Arch. Biochem. Biophys., 333, 121-126.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| cloned into the pQE expression vector, resulting in the fusion of a hexahistidine at the N-terminus, expression in Escherichia coli | Lactococcus lactis |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| E535Q | inactive enzyme | Lactococcus lactis |
| H587Q | inactive enzyme | Lactococcus lactis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| diethyl dicarbonate | - |
Lactococcus lactis |  |
| phosphoramidon | - |
Lactococcus lactis | |
| thiorphan | weak | Lactococcus lactis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0004 | - |
angiotensin I | - |
Lactococcus lactis | |
| 0.0018 | - |
Vasoactive intestinal peptide | - |
Lactococcus lactis | |
| 0.0028 | - |
adrenocorticotropic hormone | - |
Lactococcus lactis | |
| 0.005 | - |
bradykinin | - |
Lactococcus lactis | |
| 0.00654 | - |
beta-endorphin | - |
Lactococcus lactis | |
| 0.0082 | - |
dynorphin A-17 | - |
Lactococcus lactis | |
| 0.01 | - |
Substance P | - |
Lactococcus lactis | |
| 0.01 | - |
dynorphin A-13 | - |
Lactococcus lactis | |
| 0.025 | - |
dynorphin A-6 | - |
Lactococcus lactis | |
| 0.035 | - |
dynorphin A-10 | - |
Lactococcus lactis | |
| 0.04 | - |
dynorphin A-9 | - |
Lactococcus lactis | |
| 0.1 | - |
Met-enkephalin | - |
Lactococcus lactis | |
| 0.135 | - |
alpha-neoendorphin | - |
Lactococcus lactis | |
| 0.2 | - |
Leu-enkephalin | - |
Lactococcus lactis | |
| 0.298 | - |
dynorphin A-8 | - |
Lactococcus lactis | |
| 0.41 | - |
Met-enkephalin amide | - |
Lactococcus lactis | |
| 0.612 | - |
Leu-enkephalinamide | - |
Lactococcus lactis | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Lactococcus lactis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Lactococcus lactis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.203 | - |
cleavage of succinyl-Arg-Pro-Phe-His-Leu-Leu-Val-Tyr-4-methylcoumarin 7-amide | Lactococcus lactis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| adrenocorticotropic hormone + H2O | - |
Lactococcus lactis | ? | - |
? | |
| alpha-neoendorphin + H2O | - |
Lactococcus lactis | ? | - |
? | |
| angiotensin I + H2O | - |
Lactococcus lactis | ? | - |
? | |
| Arg-Pro-Lys-Pro-Gln-Gln-Phe-Phe-Gly-Leu-Met-NH2 + H2O | - |
Lactococcus lactis | ? | - |
? | |
| beta-endorphin + H2O | - |
Lactococcus lactis | ? | - |
? | |
| beta-neoendorphin + H2O | - |
Lactococcus lactis | ? | - |
? | |
| bradykinin + H2O | - |
Lactococcus lactis | ? | - |
? | |
| dynorphin + H2O | dynorphin A-6, A-8, A-9, A-10, A-13 and A-17 | Lactococcus lactis | ? | - |
? | |
| dynorphin A-10 + H2O | - |
Lactococcus lactis | ? | - |
? | |
| dynorphin A-13 + H2O | - |
Lactococcus lactis | ? | - |
? | |
| dynorphin A-17 + H2O | - |
Lactococcus lactis | ? | - |
? | |
| dynorphin A-6 + H2O | - |
Lactococcus lactis | ? | - |
? | |
| dynorphin A-8 + H2O | - |
Lactococcus lactis | ? | - |
? | |
| dynorphin A-9 + H2O | - |
Lactococcus lactis | ? | - |
? | |
| enkephalin + H2O | - |
Lactococcus lactis | ? | - |
? | |
| Leu-enkephalin + H2O | - |
Lactococcus lactis | ? | - |
? | |
| Leu5-enkephalinamide + H2O | - |
Lactococcus lactis | ? | - |
? | |
| Met-enkephalin + H2O | - |
Lactococcus lactis | ? | - |
? | |
| Met-enkephalin amide + H2O | - |
Lactococcus lactis | ? | - |
? | |
| Substance P + H2O | - |
Lactococcus lactis | ? | - |
? | |
| succinyl-Arg-Pro-Phe-His-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin + H2O | - |
Lactococcus lactis | ? | - |
? | |
| Vasoactive intestinal peptide + H2O | - |
Lactococcus lactis | ? | - |
? | |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.5 | - |
Met-enkephalin amide | - |
Lactococcus lactis | |
| 1.7 | - |
adrenocorticotropic hormone | - |
Lactococcus lactis | |
| 1.8 | - |
Vasoactive intestinal peptide | - |
Lactococcus lactis | |
| 4.3 | - |
bradykinin | - |
Lactococcus lactis | |
| 7.8 | - |
Met-enkephalin | - |
Lactococcus lactis | |
| 8.2 | - |
Leu-enkephalinamide | - |
Lactococcus lactis | |
| 14 | - |
dynorphin A-6 | - |
Lactococcus lactis | |
| 15 | - |
Leu-enkephalin | - |
Lactococcus lactis | |
| 18.6 | - |
dynorphin A-13 | - |
Lactococcus lactis | |
| 19.6 | - |
dynorphin A-8 | - |
Lactococcus lactis | |
| 23.8 | - |
dynorphin A-10 | - |
Lactococcus lactis | |
| 25.4 | - |
dynorphin A-17 | - |
Lactococcus lactis | |
| 26 | - |
beta-endorphin | - |
Lactococcus lactis | |
| 28 | - |
Substance P | - |
Lactococcus lactis | |
| 32.2 | - |
dynorphin A-9 | - |
Lactococcus lactis | |
| 44.6 | - |
alpha-neoendorphin | - |
Lactococcus lactis | |
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