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Literature summary for 3.4.24.11 extracted from

  • Chen, P.T.; Chen, C.L.; Lin, L.T.; Lo, C.H.; Hu, C.J.; Chen, R.P.; Wang, S.S.
    Design of peptide substrate for sensitively and specifically detecting two Abeta-degrading enzymes neprilysin and angiotensin-converting enzyme (2016), PLoS ONE, 11, e0153360 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.24.11

Application

Application Comment Organism
analysis the synthetic fluorogenic peptide substrate qf-Abeta(12-16)AAC is more sensitive to NEP than the previously reported peptide substrates, so that concentrations of NEP as low as 0.03 nM can be detected at peptide concentration of 0.002 mM. It can be used for high-throughput screening of compounds that upregulate NEP Homo sapiens
medicine upregulation of neprilysin (NEP) to reduce amyloid-beta (Abeta) accumulation in the brain is a promising strategy for the prevention of Alzheimer's disease (AD) Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
amyloid-beta + H2O Homo sapiens
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 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens P08473
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-

Source Tissue

Source Tissue Comment Organism Textmining
neuroblastoma cell
-
 Homo sapiens
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SH-SY5Y cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
amyloid-beta + H2O
-
 Homo sapiens ?
-
 ?
amyloid-beta(1-7)AAC + H2O a synthetic peptide, design and synthesis of a quenched fluorogenic peptide substrate qf-Abeta(1-7)AAC (with the sequence VHHQKAAC), which has a fluorophore, Alexa-350, linked to the side-chain of its C-terminal cysteine and a quencher, Dabcyl, linked to its N-terminus Homo sapiens ?
-
 ?
amyloid-beta(12-16)AAC + H2O a synthetic peptide, design and synthesis of a quenched fluorogenic peptide substrate qf-Abeta(12-16)AAC (with the sequence VHHQKAAC), which has a fluorophore, Alexa-350, linked to the side-chain of its C-terminal cysteine and a quencher, Dabcyl, linked to its N-terminus. This peptide emits strong fluorescence upon cleavage. qf-Abeta(12-16)AAC is more sensitive to NEP than the previously reported peptide substrates, so that concentrations of NEP as low as 0.03 nM can be detected at peptide concentration of 0.002 mM Homo sapiens ?
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 ?
additional information activity measurement optimization using the synthetic fluorogenic peptide substrates, evaluation and kinetics, overview Homo sapiens ?
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additional information activity measurement optimization using the synthetic fluorogenic peptide substrates, evaluation and kinetics, overview Homo sapiens ?
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 ?

Synonyms

Synonyms Comment Organism
Abeta-degrading enzyme
-
 Homo sapiens
NEP
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2 7.5 assay at Homo sapiens