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Literature summary for 3.4.24.11 extracted from

  • Hornung, K.; Zampar, S.; Engel, N.; Klafki, H.; Liepold, T.; Bayer, T.A.; Wiltfang, J.; Jahn, O.; Wirths, O.
    N-terminal truncated Abeta4-42 is a substrate for neprilysin degradation in vitro and in vivo (2019), J. Alzheimers Dis., 67, 849-858 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
recombinant viral expression of NEP in primary neurons Homo sapiens

Protein Variants

Protein Variants Comment Organism
additional information viral expression of NEP in primary neurons leads to effective clearance of amylod-beta in vitro Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens P08473
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-

Source Tissue

Source Tissue Comment Organism Textmining
commercial preparation
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Homo sapiens
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neuron CA1 neuron, NEP is almost exclusively expressed in neurons. Presynaptic terminals and intracellular locations are likely the main sites of NEP-mediated amyloid-beta degradation Homo sapiens
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
amyloid-beta1-40 + H2O a synthetic peptide Homo sapiens ?
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?
amyloid-beta1-42 + H2O a synthetic peptide Homo sapiens ?
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?
amyloid-beta4-40 + H2O a synthetic peptide Homo sapiens ?
-
?
amyloid-beta4-42 + H2O a synthetic peptide Homo sapiens ?
-
?
additional information analysis of intact amyloid-beta peptides and their NEP cleavage products by mass spectrometry. 73% of amyloid-beta4-40, 31% of amyloid-beta4-42, 45% of amyloid-beta1-40, and 18% of amyloid-beta1-42 degradation resulting from NEP catalyzed hydrolysis. Amyloid-beta4-9/amyloid-beta1-9, amyloid-beta10-17, and amyloid-beta20-30 are the major enzymatic products Homo sapiens ?
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additional information analysis of intact amyloid-beta peptides and their NEP cleavage products by mass spectrometry. 73% of amyloid-beta4-40, 31% of amyloid-beta4-42, 45% of amyloid-beta1-40, and 18% of amyloid-beta1-42 degradation resulting from NEP catalyzed hydrolysis. Amyloid-beta4-9/amyloid-beta1-9, amyloid-beta10-17, and amyloid-beta20-30 are the major enzymatic products Homo sapiens ?
-
?

Synonyms

Synonyms Comment Organism
NEP
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Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
assay at Homo sapiens

General Information

General Information Comment Organism
malfunction a lack of 465 NEP accelerates hippocampal neuron loss in relatively young transgenic mice expressing amyloid-beta4-42 Homo sapiens
physiological function N-terminal truncated Abeta4-42 is a substrate for neprilysin degradation in vitro and in vivo Homo sapiens