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Literature summary for 3.4.24.11 extracted from
- Recombinant soluble neprilysin reduces amyloid-beta accumulation and improves memory impairment in Alzheimers disease mice (2013), Brain Res., 1529, 113-124.
Application
| Application | Comment | Organism |
|---|---|---|
| medicine | the enzyme is a potential therapeutic source for degradation of deposited amyloid beta in Alzheimer's disease | Homo sapiens |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| cloning of the extracellular enzyme domain (52-750) with a is-tag and a gp64 signal sequence in pBAC-6 expression vector in Spodoptera frugiperda insect cells and recombinant expression of the His-tagged enzyme in insect cells and in Alzheimer disease mice using intracerebral injection of the NEP-expression vector. Accumulation of amyloid beta is significantly reduced and the behavioral performance of the mice is improved, phenotype, overview | Homo sapiens |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| plasma membrane | - |
Homo sapiens | 5886 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Zn2+ | metalloprotease | Homo sapiens |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 84000 | - |
x * 84000, recombinant soluble screted His-tagged enzyme, SDS-PAGE | Homo sapiens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| amyloid beta peptide + H2O | Homo sapiens | - |
? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | P08473 | - |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | - |
Homo sapiens |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant soluble screted His-tagged enzyme from Sf9 insect cells by affinity chromatography and gel filtration | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | the enzyme level is reduced in the brains of patients with Alzheimer's disease | Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| amyloid beta peptide + H2O | - |
Homo sapiens | ? | - |
? | |
| glutaryl-Ala-Ala-Phe-4-methoxy-2-naphthylamide + H2O | - |
Homo sapiens | glutaryl-Ala-Ala + Phe-4-methoxy-2-naphthylamide | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 84000, recombinant soluble screted His-tagged enzyme, SDS-PAGE | Homo sapiens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| NEP | - |
Homo sapiens |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | neprilysin is a plasma membrane glycoprotein of the neutral zinc metalloendopeptidase family | Homo sapiens |
| physiological function | amyloid beta-induced death of hippocampal neuron of APP/PS1 mice is inhibited by human recombinant soluble enzyme, overview | Homo sapiens |
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