BRENDA - Enzyme Database
show all sequences of 3.4.23.B9

Bovine leukemia virus retropepsin

Menard, A.; Guillemain, B.; Handbook of Proteolytic Enzymes (Barrett, J. ; Rawlings, N. D. ; Woessner, J. F. , eds. ) 1, 169-171 (2004)
No PubMed abstract available

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
expression in Escherichia coli
bovine leukemia virus
Inhibitors
Inhibitors
Commentary
Organism
Structure
Ac-Pro-Glu-Val-Leu-azetidine-Val-Met
IC50: 0.250 mM
bovine leukemia virus
Ac-Pro-Glu-Val-Leu-pipecolic acid-Val-Met
IC50: 0.015 mM
bovine leukemia virus
cerulenin
IC50: 5 mM
bovine leukemia virus
Cu2+
-
bovine leukemia virus
Gly-Val-Leu-Tyr-statine-Glu-Ala
IC50: 0.10 mM
bovine leukemia virus
pepstatin A
IC50: 0.3-0.5 mM
bovine leukemia virus
Pro-Glu-Val-statine-Ala-Leu
IC50: 0.10 mM
bovine leukemia virus
YDPPAI-statine-II
strong inhibition, IC50: 0.0005 mM
bovine leukemia virus
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
ammonium sulfate
activates
bovine leukemia virus
NaCl
activates at 1-2 M
bovine leukemia virus
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
14000
-
2 * 14000
bovine leukemia virus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
Gag polyprotein + H2O
bovine leukemia virus
processing of the precursor protein into mature structural proteins of the matrix, capsid, and nucleocapsid, and the functional protease
?
-
-
?
Gag polyprotein + H2O
bovine leukemia virus BLV
processing of the precursor protein into mature structural proteins of the matrix, capsid, and nucleocapsid, and the functional protease
?
-
-
?
Gag-Pol polyprotein + H2O
bovine leukemia virus
processing of the precursor protein into mature structural proteins of the matrix, capsid, and nucleocapsid, and the functional protease
?
-
-
?
Gag-Pol polyprotein + H2O
bovine leukemia virus BLV
processing of the precursor protein into mature structural proteins of the matrix, capsid, and nucleocapsid, and the functional protease
?
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
bovine leukemia virus
-
BLV
-
bovine leukemia virus BLV
-
BLV
-
Posttranslational Modification
Posttranslational Modification
Commentary
Organism
proteolytic modification
autolytic processing, the enzyme can hydrolyze its own N- and C-terminals, e.g. by cleaving the C-terminal site ASISI-/-PEEV during storage
bovine leukemia virus
Purification (Commentary)
Purification (Commentary)
Organism
native enzyme from viral particles under denaturing conditions, and recombinant enzyme from Escherichia coli
bovine leukemia virus
Reaction
Reaction
Commentary
Organism
Reaction ID
The best substrate YDPPAILPII is bearing the natural cleavage site between the matrix and the capsid proteins of BLV Gag precursor. polyprotein. Good cleavage of the peptide bonds: Leu-Pro, Leu-Val, Gly-Val and Leu-Pro
the enzyme contains the aspartic protease sequence Asp-Thr-Gly
bovine leukemia virus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
Gag polyprotein + H2O
-
668796
bovine leukemia virus
?
-
-
-
?
Gag polyprotein + H2O
processing of the precursor protein into mature structural proteins of the matrix, capsid, and nucleocapsid, and the functional protease
668796
bovine leukemia virus
?
-
-
-
?
Gag polyprotein + H2O
-
668796
bovine leukemia virus BLV
?
-
-
-
?
Gag polyprotein + H2O
processing of the precursor protein into mature structural proteins of the matrix, capsid, and nucleocapsid, and the functional protease
668796
bovine leukemia virus BLV
?
-
-
-
?
Gag-Pol polyprotein + H2O
-
668796
bovine leukemia virus
?
-
-
-
?
Gag-Pol polyprotein + H2O
processing of the precursor protein into mature structural proteins of the matrix, capsid, and nucleocapsid, and the functional protease
668796
bovine leukemia virus
?
-
-
-
?
Gag-Pol polyprotein + H2O
-
668796
bovine leukemia virus BLV
?
-
-
-
?
Gag-Pol polyprotein + H2O
processing of the precursor protein into mature structural proteins of the matrix, capsid, and nucleocapsid, and the functional protease
668796
bovine leukemia virus BLV
?
-
-
-
?
YDPPAILPII + H2O
represents the CA/NC cleavage site of the Gag polyprotein
668796
bovine leukemia virus
YDPPAIL + Pro-Ile-Ile
-
-
-
?
YDPPAILPII + H2O
represents the CA/NC cleavage site of the Gag polyprotein
668796
bovine leukemia virus BLV
YDPPAIL + Pro-Ile-Ile
-
-
-
?
Subunits
Subunits
Commentary
Organism
dimer
2 * 14000
bovine leukemia virus
Synonyms
Synonyms
Commentary
Organism
BLV endopeptidase
-
bovine leukemia virus
BLV protease
-
bovine leukemia virus
bovine leukemia virus retropepsin
-
bovine leukemia virus
More
the enzyme belongs to the A2 peptidase family
bovine leukemia virus
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
40
-
-
bovine leukemia virus
Temperature Range [°C]
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
-
80
active range
bovine leukemia virus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5
6
-
bovine leukemia virus
pH Range
pH Minimum
pH Maximum
Commentary
Organism
3.5
7.5
active range
bovine leukemia virus
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
5
6.5
the purified enzyme is more stable at pH 5.0 than at pH 6.5 due to the reduced autolytic activity at the C-terminus
bovine leukemia virus
pI Value
Organism
Commentary
pI Value Maximum
pI Value
bovine leukemia virus
-
-
10.2
IC50 Value
IC50 Value
IC50 Value Maximum
Commentary
Organism
Inhibitor
Structure
0.0005
-
strong inhibition, IC50: 0.0005 mM
bovine leukemia virus
YDPPAI-statine-II
0.015
-
IC50: 0.015 mM
bovine leukemia virus
Ac-Pro-Glu-Val-Leu-pipecolic acid-Val-Met
0.1
-
IC50: 0.10 mM
bovine leukemia virus
Gly-Val-Leu-Tyr-statine-Glu-Ala
0.1
-
IC50: 0.10 mM
bovine leukemia virus
Pro-Glu-Val-statine-Ala-Leu
0.25
-
IC50: 0.250 mM
bovine leukemia virus
Ac-Pro-Glu-Val-Leu-azetidine-Val-Met
0.3
0.5
IC50: 0.3-0.5 mM
bovine leukemia virus
pepstatin A
5
-
IC50: 5 mM
bovine leukemia virus
cerulenin
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli
bovine leukemia virus
IC50 Value (protein specific)
IC50 Value
IC50 Value Maximum
Commentary
Organism
Inhibitor
Structure
0.0005
-
strong inhibition, IC50: 0.0005 mM
bovine leukemia virus
YDPPAI-statine-II
0.015
-
IC50: 0.015 mM
bovine leukemia virus
Ac-Pro-Glu-Val-Leu-pipecolic acid-Val-Met
0.1
-
IC50: 0.10 mM
bovine leukemia virus
Gly-Val-Leu-Tyr-statine-Glu-Ala
0.1
-
IC50: 0.10 mM
bovine leukemia virus
Pro-Glu-Val-statine-Ala-Leu
0.25
-
IC50: 0.250 mM
bovine leukemia virus
Ac-Pro-Glu-Val-Leu-azetidine-Val-Met
0.3
0.5
IC50: 0.3-0.5 mM
bovine leukemia virus
pepstatin A
5
-
IC50: 5 mM
bovine leukemia virus
cerulenin
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Ac-Pro-Glu-Val-Leu-azetidine-Val-Met
IC50: 0.250 mM
bovine leukemia virus
Ac-Pro-Glu-Val-Leu-pipecolic acid-Val-Met
IC50: 0.015 mM
bovine leukemia virus
cerulenin
IC50: 5 mM
bovine leukemia virus
Cu2+
-
bovine leukemia virus
Gly-Val-Leu-Tyr-statine-Glu-Ala
IC50: 0.10 mM
bovine leukemia virus
pepstatin A
IC50: 0.3-0.5 mM
bovine leukemia virus
Pro-Glu-Val-statine-Ala-Leu
IC50: 0.10 mM
bovine leukemia virus
YDPPAI-statine-II
strong inhibition, IC50: 0.0005 mM
bovine leukemia virus
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
ammonium sulfate
activates
bovine leukemia virus
NaCl
activates at 1-2 M
bovine leukemia virus
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
14000
-
2 * 14000
bovine leukemia virus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
Gag polyprotein + H2O
bovine leukemia virus
processing of the precursor protein into mature structural proteins of the matrix, capsid, and nucleocapsid, and the functional protease
?
-
-
?
Gag polyprotein + H2O
bovine leukemia virus BLV
processing of the precursor protein into mature structural proteins of the matrix, capsid, and nucleocapsid, and the functional protease
?
-
-
?
Gag-Pol polyprotein + H2O
bovine leukemia virus
processing of the precursor protein into mature structural proteins of the matrix, capsid, and nucleocapsid, and the functional protease
?
-
-
?
Gag-Pol polyprotein + H2O
bovine leukemia virus BLV
processing of the precursor protein into mature structural proteins of the matrix, capsid, and nucleocapsid, and the functional protease
?
-
-
?
Posttranslational Modification (protein specific)
Posttranslational Modification
Commentary
Organism
proteolytic modification
autolytic processing, the enzyme can hydrolyze its own N- and C-terminals, e.g. by cleaving the C-terminal site ASISI-/-PEEV during storage
bovine leukemia virus
Purification (Commentary) (protein specific)
Commentary
Organism
native enzyme from viral particles under denaturing conditions, and recombinant enzyme from Escherichia coli
bovine leukemia virus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
Gag polyprotein + H2O
-
668796
bovine leukemia virus
?
-
-
-
?
Gag polyprotein + H2O
processing of the precursor protein into mature structural proteins of the matrix, capsid, and nucleocapsid, and the functional protease
668796
bovine leukemia virus
?
-
-
-
?
Gag polyprotein + H2O
-
668796
bovine leukemia virus BLV
?
-
-
-
?
Gag polyprotein + H2O
processing of the precursor protein into mature structural proteins of the matrix, capsid, and nucleocapsid, and the functional protease
668796
bovine leukemia virus BLV
?
-
-
-
?
Gag-Pol polyprotein + H2O
-
668796
bovine leukemia virus
?
-
-
-
?
Gag-Pol polyprotein + H2O
processing of the precursor protein into mature structural proteins of the matrix, capsid, and nucleocapsid, and the functional protease
668796
bovine leukemia virus
?
-
-
-
?
Gag-Pol polyprotein + H2O
-
668796
bovine leukemia virus BLV
?
-
-
-
?
Gag-Pol polyprotein + H2O
processing of the precursor protein into mature structural proteins of the matrix, capsid, and nucleocapsid, and the functional protease
668796
bovine leukemia virus BLV
?
-
-
-
?
YDPPAILPII + H2O
represents the CA/NC cleavage site of the Gag polyprotein
668796
bovine leukemia virus
YDPPAIL + Pro-Ile-Ile
-
-
-
?
YDPPAILPII + H2O
represents the CA/NC cleavage site of the Gag polyprotein
668796
bovine leukemia virus BLV
YDPPAIL + Pro-Ile-Ile
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
2 * 14000
bovine leukemia virus
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
40
-
-
bovine leukemia virus
Temperature Range [°C] (protein specific)
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
-
80
active range
bovine leukemia virus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5
6
-
bovine leukemia virus
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
3.5
7.5
active range
bovine leukemia virus
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
5
6.5
the purified enzyme is more stable at pH 5.0 than at pH 6.5 due to the reduced autolytic activity at the C-terminus
bovine leukemia virus
pI Value (protein specific)
Organism
Commentary
pI Value Maximum
pI Value
bovine leukemia virus
-
-
10.2
Other publictions for EC 3.4.23.B9
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
693905
Eizert
Amino acid preferences of retr ...
bovine leukemia virus
J. Virol.
82
10111-10117
2008
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
7
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
7
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
681171
Sperka
Bovine leukemia virus protease ...
bovine leukemia virus
J. Gen. Virol.
88
2052-2063
2007
2
-
-
-
4
-
14
43
-
-
-
-
-
6
-
-
-
-
-
-
-
-
40
-
2
1
-
-
44
1
-
-
-
14
-
-
2
-
-
-
-
4
-
-
14
14
43
-
-
-
-
-
-
-
-
-
-
-
-
40
-
1
-
-
44
1
-
-
-
-
-
-
-
-
-
666190
Bagossi
Amino acid preferences for a c ...
bovine leukemia virus
J. Virol.
79
4213-4218
2005
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
1
-
-
-
-
-
10
1
2
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
10
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
668796
Menard
-
Bovine leukemia virus retropep ...
bovine leukemia virus, bovine leukemia virus BLV
Handbook of Proteolytic Enzymes (Barrett, J. ; Rawlings, N. D. ; Woessner, J. F. , eds. )
1
169-171
2004
-
-
1
-
-
-
8
-
-
2
1
4
-
2
-
1
1
1
-
-
-
-
10
1
4
1
1
-
-
1
1
1
-
-
1
7
-
-
1
-
-
-
-
7
8
-
-
-
2
1
4
-
-
1
1
-
-
-
-
10
1
1
1
-
-
1
1
1
1
-
-
-
-
-
-
647867
Zahuczky
Cloning of the bovine leukemia ...
bovine leukemia virus
Biochim. Biophys. Acta
1478
1-8
2000
-
-
1
-
-
-
-
2
1
-
-
-
-
1
-
-
1
-
-
-
-
-
6
-
-
-
-
-
6
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
1
-
-
-
-
6
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
647864
Menard
Inhibition of activity of the ...
bovine leukemia virus
FEBS Lett.
346
268-272
1994
-
-
-
-
-
-
5
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
5
5
-
-
-
-
-
1
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
647865
Precigoux
Modelling, synthesis and biolo ...
bovine leukemia virus
FEBS Lett.
326
237-240
1993
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
647866
Menard
Bovine leukemia virus: purific ...
bovine leukemia virus
Virology
193
680-689
1993
-
-
-
-
-
-
1
-
-
2
1
-
-
3
-
-
1
-
-
-
1
-
1
1
-
1
1
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
2
1
-
-
-
-
1
-
-
1
-
1
1
1
1
-
-
1
1
-
-
-
-
-
-
-
-
647863
Rice N.R.; Stephens R.M.; Burny A.; Gilden R.V.
The gag and pol genes of bovin ...
bovine leukemia virus
Virology
142
357-377
1985
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-