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Literature summary for 3.4.23.B9 extracted from

  • Zahuczky, G.; Boross, P.; Bagossi, P.; Emri, G.; Copeland, T.D.; Oroszlan, S.; Louis, J.M.; Tozser, J.
    Cloning of the bovine leukemia virus proteinase in Escherichia coli and comparison of its specificity to that of human T-cell leukemia virus proteinase (2000), Biochim. Biophys. Acta, 1478, 1-8.
    View publication on PubMed
Search for more literature for 3.4.23.B9

Cloned(Commentary)

Cloned (Comment) Organism
the enzyme is cloned into pMal-c2 vector with N-terminal or with N- as well as C-terminal flanking sequences, and expressed in fusion with maltose binding protein. The proteinase self-processes itself from the fusion protein during expression and forms inclusion bodies bovine leukemia virus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.019
-
 KGPPVILPIQAP pH 5.6, 37°C bovine leukemia virus
0.023
-
 PPMVGVLDAP pH 5.6, 37°C bovine leukemia virus

Localization

Localization Comment Organism GeneOntology No. Textmining
additional information enzyme is purified from inclusion bodies of Escherichia coli bovine leukemia virus
-
-

Organism

Organism UniProt Comment Textmining
bovine leukemia virus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 bovine leukemia virus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
APQVLPVMHP + H2O
-
 bovine leukemia virus APQVL + PVMHP
-
 ?
KGPPVILPIQAP + H2O
-
 bovine leukemia virus KGPPVIL + PIQAP
-
 ?
KQPAILVHTPG + H2O
-
 bovine leukemia virus KQPAIL + VHTPG
-
 ?
KTKVLVVQPK + H2O
-
 bovine leukemia virus KTKVL + VVQPK
-
 ?
PPAILPIISE + H2O
-
 bovine leukemia virus PPAIL + PIISE
-
 ?
PPMVGVLDAP + H2O
-
 bovine leukemia virus PPMVG + VLDAP
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.01
-
 PPMVGVLDAP pH 5.6, 37°C bovine leukemia virus
0.02
-
 KGPPVILPIQAP pH 5.6, 37°C bovine leukemia virus
0.04
-
 PPAILPIISE pH 5.6, 37°C bovine leukemia virus
0.07
-
 APQVLPVMHP pH 5.6, 37°C bovine leukemia virus
0.17
-
 KTKVLVVQPK pH 5.6, 37°C bovine leukemia virus
0.18
-
 KQPAILVHTPG pH 5.6, 37°C bovine leukemia virus