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show all sequences of 3.4.23.B9

Cloning of the bovine leukemia virus proteinase in Escherichia coli and comparison of its specificity to that of human T-cell leukemia virus proteinase

Zahuczky, G.; Boross, P.; Bagossi, P.; Emri, G.; Copeland, T.D.; Oroszlan, S.; Louis, J.M.; Tozser, J.; Biochim. Biophys. Acta 1478, 1-8 (2000)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
the enzyme is cloned into pMal-c2 vector with N-terminal or with N- as well as C-terminal flanking sequences, and expressed in fusion with maltose binding protein. The proteinase self-processes itself from the fusion protein during expression and forms inclusion bodies
bovine leukemia virus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.019
-
KGPPVILPIQAP
pH 5.6, 37°C
bovine leukemia virus
0.023
-
PPMVGVLDAP
pH 5.6, 37°C
bovine leukemia virus
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
additional information
enzyme is purified from inclusion bodies of Escherichia coli
bovine leukemia virus
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
bovine leukemia virus
-
-
-
Purification (Commentary)
Commentary
Organism
-
bovine leukemia virus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
APQVLPVMHP + H2O
-
647867
bovine leukemia virus
APQVL + PVMHP
-
647867
bovine leukemia virus
?
KGPPVILPIQAP + H2O
-
647867
bovine leukemia virus
KGPPVIL + PIQAP
-
647867
bovine leukemia virus
?
KQPAILVHTPG + H2O
-
647867
bovine leukemia virus
KQPAIL + VHTPG
-
647867
bovine leukemia virus
?
KTKVLVVQPK + H2O
-
647867
bovine leukemia virus
KTKVL + VVQPK
-
647867
bovine leukemia virus
?
PPAILPIISE + H2O
-
647867
bovine leukemia virus
PPAIL + PIISE
-
647867
bovine leukemia virus
?
PPMVGVLDAP + H2O
-
647867
bovine leukemia virus
PPMVG + VLDAP
-
647867
bovine leukemia virus
?
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.01
-
PPMVGVLDAP
pH 5.6, 37°C
bovine leukemia virus
0.02
-
KGPPVILPIQAP
pH 5.6, 37°C
bovine leukemia virus
0.04
-
PPAILPIISE
pH 5.6, 37°C
bovine leukemia virus
0.07
-
APQVLPVMHP
pH 5.6, 37°C
bovine leukemia virus
0.17
-
KTKVLVVQPK
pH 5.6, 37°C
bovine leukemia virus
0.18
-
KQPAILVHTPG
pH 5.6, 37°C
bovine leukemia virus
Cloned(Commentary) (protein specific)
Commentary
Organism
the enzyme is cloned into pMal-c2 vector with N-terminal or with N- as well as C-terminal flanking sequences, and expressed in fusion with maltose binding protein. The proteinase self-processes itself from the fusion protein during expression and forms inclusion bodies
bovine leukemia virus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.019
-
KGPPVILPIQAP
pH 5.6, 37°C
bovine leukemia virus
0.023
-
PPMVGVLDAP
pH 5.6, 37°C
bovine leukemia virus
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
additional information
enzyme is purified from inclusion bodies of Escherichia coli
bovine leukemia virus
-
-
Purification (Commentary) (protein specific)
Commentary
Organism
-
bovine leukemia virus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
APQVLPVMHP + H2O
-
647867
bovine leukemia virus
APQVL + PVMHP
-
647867
bovine leukemia virus
?
KGPPVILPIQAP + H2O
-
647867
bovine leukemia virus
KGPPVIL + PIQAP
-
647867
bovine leukemia virus
?
KQPAILVHTPG + H2O
-
647867
bovine leukemia virus
KQPAIL + VHTPG
-
647867
bovine leukemia virus
?
KTKVLVVQPK + H2O
-
647867
bovine leukemia virus
KTKVL + VVQPK
-
647867
bovine leukemia virus
?
PPAILPIISE + H2O
-
647867
bovine leukemia virus
PPAIL + PIISE
-
647867
bovine leukemia virus
?
PPMVGVLDAP + H2O
-
647867
bovine leukemia virus
PPMVG + VLDAP
-
647867
bovine leukemia virus
?
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.01
-
PPMVGVLDAP
pH 5.6, 37°C
bovine leukemia virus
0.02
-
KGPPVILPIQAP
pH 5.6, 37°C
bovine leukemia virus
0.04
-
PPAILPIISE
pH 5.6, 37°C
bovine leukemia virus
0.07
-
APQVLPVMHP
pH 5.6, 37°C
bovine leukemia virus
0.17
-
KTKVLVVQPK
pH 5.6, 37°C
bovine leukemia virus
0.18
-
KQPAILVHTPG
pH 5.6, 37°C
bovine leukemia virus
Other publictions for EC 3.4.23.B9
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
693905
Eizert
Amino acid preferences of retr ...
bovine leukemia virus
J. Virol.
82
10111-10117
2008
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-
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1
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-
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7
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7
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-
681171
Sperka
Bovine leukemia virus protease ...
bovine leukemia virus
J. Gen. Virol.
88
2052-2063
2007
2
-
-
-
4
-
14
43
-
-
-
-
-
6
-
-
-
-
-
-
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-
40
-
1
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-
44
1
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-
14
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-
2
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-
-
-
4
-
-
14
14
43
-
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-
-
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-
-
-
-
-
-
-
40
-
1
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44
1
-
-
-
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-
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-
666190
Bagossi
Amino acid preferences for a c ...
bovine leukemia virus
J. Virol.
79
4213-4218
2005
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-
-
-
-
-
-
-
1
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-
-
1
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-
1
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10
1
1
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-
1
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-
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-
-
-
-
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-
1
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-
1
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-
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-
10
1
1
-
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-
1
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-
-
-
-
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-
-
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668796
Menard
-
Bovine leukemia virus retropep ...
bovine leukemia virus, bovine leukemia virus BLV
Handbook of Proteolytic Enzymes (Barrett, J. ; Rawlings, N. D. ; Woessner, J. F. , eds. )
1
169-171
2004
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-
1
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-
-
8
-
-
2
1
4
-
2
-
1
1
1
-
-
-
-
10
1
1
1
-
-
1
1
1
-
-
1
7
-
-
1
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-
7
8
-
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-
2
1
4
-
-
1
1
-
-
-
-
10
1
1
1
-
-
1
1
1
1
-
-
-
-
-
-
647867
Zahuczky
Cloning of the bovine leukemia ...
bovine leukemia virus
Biochim. Biophys. Acta
1478
1-8
2000
-
-
1
-
-
-
-
2
1
-
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-
-
1
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-
1
-
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-
-
-
6
-
-
-
-
6
-
-
-
-
-
-
-
-
-
1
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-
-
-
-
-
2
1
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-
1
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-
6
-
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-
6
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647864
Menard
Inhibition of activity of the ...
bovine leukemia virus
FEBS Lett.
346
268-272
1994
-
-
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-
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-
5
-
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1
-
1
-
-
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-
-
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-
5
-
-
-
-
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-
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-
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-
-
5
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-
-
-
-
5
5
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-
1
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-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
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-
-
-
-
-
-
647865
Precigoux
Modelling, synthesis and biolo ...
bovine leukemia virus
FEBS Lett.
326
237-240
1993
-
-
-
-
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1
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-
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1
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1
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1
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1
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647866
Menard
Bovine leukemia virus: purific ...
bovine leukemia virus
Virology
193
680-689
1993
-
-
-
-
-
-
1
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-
2
1
-
-
3
-
-
1
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-
1
-
1
1
1
1
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-
1
1
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-
1
-
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2
1
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1
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1
-
1
1
1
1
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-
1
1
-
-
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-
-
-
-
-
647863
Rice N.R.; Stephens R.M.; Burny A.; Gilden R.V.
The gag and pol genes of bovin ...
bovine leukemia virus
Virology
142
357-377
1985
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1
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