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show all sequences of 3.4.23.B9

Bovine leukemia virus: purification and characterization of the aspartic protease

Menard, A.; Mamoun, R.Z.; Geoffre, S.; Castroviejo, M.; Raymond, S.; Precigoux, G.; Hospital, M.; Guillemain, B.; Virology 193, 680-689 (1993)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
YDPPAI-statine-II
-
bovine leukemia virus
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
(NH4)2SO4
with YDPPAILPII as substrate, most active at 1-2 M NaCl or (NH4)2SO4
bovine leukemia virus
NaCl
with YDPPAILPII as substrate, most active at 1-2 M NaCl or (NH4)2SO4. With human T-cell leukemia virus recombinant gag precursor activity is higher at 0.5 M NaCl
bovine leukemia virus
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
14000
-
2 * 14000, SDS-PAGE
bovine leukemia virus
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
bovine leukemia virus
-
-
-
Purification (Commentary)
Commentary
Organism
-
bovine leukemia virus
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.25
-
reaction with YDPPAILPII
bovine leukemia virus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
YDPPAILPII + H2O
best substrate, bearing the natural cleavage site between the matrix and the capsid proteins of BLV Gag precursor polyprotein
647866
bovine leukemia virus
YDPPAIL + PII
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
2 * 14000, SDS-PAGE
bovine leukemia virus
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
40
-
reaction with YDPPAILPII
bovine leukemia virus
Temperature Range [°C]
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
20
80
20°C: about 45% of maximal activity, 80°C: about 70% of maximal activity, reaction with YDPPAILPII
bovine leukemia virus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5
6
reaction with YDPPAILPII
bovine leukemia virus
pH Range
pH Minimum
pH Maximum
Commentary
Organism
3.5
7.5
pH 3.5: about 90% of maximal activity, pH 7.5: about 70% of maximal activity, reaction with YDPPAILPII
bovine leukemia virus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
YDPPAI-statine-II
-
bovine leukemia virus
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
(NH4)2SO4
with YDPPAILPII as substrate, most active at 1-2 M NaCl or (NH4)2SO4
bovine leukemia virus
NaCl
with YDPPAILPII as substrate, most active at 1-2 M NaCl or (NH4)2SO4. With human T-cell leukemia virus recombinant gag precursor activity is higher at 0.5 M NaCl
bovine leukemia virus
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
14000
-
2 * 14000, SDS-PAGE
bovine leukemia virus
Purification (Commentary) (protein specific)
Commentary
Organism
-
bovine leukemia virus
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.25
-
reaction with YDPPAILPII
bovine leukemia virus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
YDPPAILPII + H2O
best substrate, bearing the natural cleavage site between the matrix and the capsid proteins of BLV Gag precursor polyprotein
647866
bovine leukemia virus
YDPPAIL + PII
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
2 * 14000, SDS-PAGE
bovine leukemia virus
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
40
-
reaction with YDPPAILPII
bovine leukemia virus
Temperature Range [°C] (protein specific)
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
20
80
20°C: about 45% of maximal activity, 80°C: about 70% of maximal activity, reaction with YDPPAILPII
bovine leukemia virus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5
6
reaction with YDPPAILPII
bovine leukemia virus
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
3.5
7.5
pH 3.5: about 90% of maximal activity, pH 7.5: about 70% of maximal activity, reaction with YDPPAILPII
bovine leukemia virus
Other publictions for EC 3.4.23.B9
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
693905
Eizert
Amino acid preferences of retr ...
bovine leukemia virus
J. Virol.
82
10111-10117
2008
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-
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-
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-
-
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1
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7
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7
-
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-
-
-
-
-
-
-
-
-
-
-
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-
681171
Sperka
Bovine leukemia virus protease ...
bovine leukemia virus
J. Gen. Virol.
88
2052-2063
2007
2
-
-
-
4
-
14
43
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-
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-
6
-
-
-
-
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-
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40
-
1
-
-
44
1
-
-
-
14
-
-
2
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-
-
-
4
-
-
14
14
43
-
-
-
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-
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-
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-
-
40
-
1
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-
44
1
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-
666190
Bagossi
Amino acid preferences for a c ...
bovine leukemia virus
J. Virol.
79
4213-4218
2005
-
-
-
-
-
-
-
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1
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1
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-
1
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10
1
1
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1
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1
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1
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10
1
1
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1
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668796
Menard
-
Bovine leukemia virus retropep ...
bovine leukemia virus, bovine leukemia virus BLV
Handbook of Proteolytic Enzymes (Barrett, J. ; Rawlings, N. D. ; Woessner, J. F. , eds. )
1
169-171
2004
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-
1
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8
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2
1
4
-
2
-
1
1
1
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10
1
1
1
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1
1
1
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1
7
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1
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7
8
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2
1
4
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1
1
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-
10
1
1
1
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1
1
1
1
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647867
Zahuczky
Cloning of the bovine leukemia ...
bovine leukemia virus
Biochim. Biophys. Acta
1478
1-8
2000
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1
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2
1
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1
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1
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6
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6
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1
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2
1
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1
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6
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6
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647864
Menard
Inhibition of activity of the ...
bovine leukemia virus
FEBS Lett.
346
268-272
1994
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5
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1
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1
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5
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5
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5
5
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1
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5
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647865
Precigoux
Modelling, synthesis and biolo ...
bovine leukemia virus
FEBS Lett.
326
237-240
1993
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1
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1
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1
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1
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1
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647866
Menard
Bovine leukemia virus: purific ...
bovine leukemia virus
Virology
193
680-689
1993
-
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1
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2
1
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3
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1
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1
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1
1
1
1
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1
1
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1
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2
1
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1
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1
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1
1
1
1
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1
1
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647863
Rice N.R.; Stephens R.M.; Burny A.; Gilden R.V.
The gag and pol genes of bovin ...
bovine leukemia virus
Virology
142
357-377
1985
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1
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