Protein Variants | Comment | Organism |
---|---|---|
L49I | autoproteolysis resistant mutant enzyme. KM-values and turnover-numbers are comparable to that of wild-type enzyme | Human T-cell leukemia virus type I |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.03 | - |
KGPPVILPIQAP | pH 5.6, 37°C, wild-type enzyme | Human T-cell leukemia virus type I | |
0.032 | - |
KTKVLVVQPK | pH 5.6, 37°C, mutant enzyme L40I | Human T-cell leukemia virus type I | |
0.033 | - |
KTKVLVVQPK | pH 5.6, 37°C, wild-type enzyme | Human T-cell leukemia virus type I | |
0.034 | - |
KGPPVILPIQAP | pH 5.6, 37°C, mutant enzyme L40I | Human T-cell leukemia virus type I | |
0.098 | - |
APQVLPVMHP | pH 5.6, 37°C, mutant enzyme L40I | Human T-cell leukemia virus type I | |
0.115 | - |
APQVLPVMHP | pH 5.6, 37°C, wild-type enzyme | Human T-cell leukemia virus type I | |
0.23 | - |
DPASILPVIP | pH 5.6, 37°C, wild-type enzyme | Human T-cell leukemia virus type I | |
0.244 | - |
DPASILPVIP | pH 5.6, 37°C, mutant enzyme L40I | Human T-cell leukemia virus type I |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Human T-cell leukemia virus type I | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
additional information | formation of a disulfide bridge, reversible oxidative modification as a mechanism for regulating retroviral protease dimerization and activation, regulation through reversible glutathionylation of its two conserved cysteine residues | Human T-cell leukemia virus type I |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
APQVLPVMHP + H2O | - |
Human T-cell leukemia virus type I | APQVL + PVMHP | - |
? | |
DPASILPVIP + H2O | - |
Human T-cell leukemia virus type I | DPASIL + PVIP | - |
? | |
KTKVLVVQPK + H2O | - |
Human T-cell leukemia virus type I | KTKVL + VVQPK | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.62 | - |
DPASILPVIP | pH 5.6, 37°C, mutant enzyme L40I | Human T-cell leukemia virus type I | |
0.7 | - |
DPASILPVIP | pH 5.6, 37°C, wild-type enzyme | Human T-cell leukemia virus type I | |
5.15 | - |
KTKVLVVQPK | pH 5.6, 37°C, wild-type enzyme | Human T-cell leukemia virus type I | |
5.18 | - |
KTKVLVVQPK | pH 5.6, 37°C, mutant enzyme L40I | Human T-cell leukemia virus type I | |
6.26 | - |
KGPPVILPIQAP | pH 5.6, 37°C, mutant enzyme L40I | Human T-cell leukemia virus type I | |
6.78 | - |
APQVLPVMHP | pH 5.6, 37°C, mutant enzyme L40I | Human T-cell leukemia virus type I | |
6.91 | - |
KGPPVILPIQAP | pH 5.6, 37°C, wild-type enzyme | Human T-cell leukemia virus type I | |
8.67 | - |
APQVLPVMHP | pH 5.6, 37°C, wild-type enzyme | Human T-cell leukemia virus type I |