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Literature summary for 3.4.23.B5 extracted from
- Moloney murine leukemia virus protease: bacterial expression and characterization of the purified enzyme (1993), Virology, 196, 557-563.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| cloned into the prokaryotic expression vector pGEX-2T, expression in fusion with the glutathione S-transferase from Schistosoma japonicum | Moloney murine leukemia virus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| acetyl-pepstatin A | IC50: 0.025 mM | Moloney murine leukemia virus |  |
| KH164 | 0.062 mM | Moloney murine leukemia virus | |
| UK88947 | IC50: 0.0022 mM | Moloney murine leukemia virus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0166 | - |
PLQVLTLNIERR | pH 6.0, 37°C | Moloney murine leukemia virus | |
| 0.0192 | - |
LTFTFPVVFMRR | pH 6.0, 37°C | Moloney murine leukemia virus | |
| 0.0242 | - |
VQALVLTQ | pH 6.0, 37°C | Moloney murine leukemia virus | |
| 0.0299 | - |
MSKLLATVVS | pH 6.0, 37°C | Moloney murine leukemia virus | |
| 0.039 | - |
PPAILPIISE | pH 6.0, 37°C | Moloney murine leukemia virus | |
| 0.0435 | - |
SDLVLLSAEARR | pH 6.0, 37°C | Moloney murine leukemia virus | |
| 0.0445 | - |
TQTSLLTLDDQ | pH 6.0, 37°C | Moloney murine leukemia virus | |
| 0.068 | - |
TSTLLIENSS | pH 6.0, 37°C | Moloney murine leukemia virus | |
| 0.0804 | - |
PRSSLYPALTP | pH 6.0, 37°C | Moloney murine leukemia virus | |
| 0.0846 | - |
DELILPVKRK | pH 6.0, 37°C | Moloney murine leukemia virus | |
| 0.098 | - |
TSQAFPLRAG | pH 6.0, 37°C | Moloney murine leukemia virus | |
| 0.121 | - |
PSEEYPIMID | pH 6.0, 37°C | Moloney murine leukemia virus | |
| 0.15 | - |
APQVLPVMHP | pH 6.0, 37°C | Moloney murine leukemia virus | |
| 0.166 | - |
VGFAGAMAEAR | pH 6.0, 37°C | Moloney murine leukemia virus | |
| 0.2 | - |
DSKAFLATDW | pH 6.0, 37°C | Moloney murine leukemia virus | |
| 0.261 | - |
VSQNYPIVQ | - |
Moloney murine leukemia virus | |
| 0.51 | - |
PKDIFPVTET | pH 6.0, 37°C | Moloney murine leukemia virus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| NaCl | optimal concentration is 3 M | Moloney murine leukemia virus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Moloney murine leukemia virus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme in fusion with the glutathione S-transferase from Schistosoma japonicum | Moloney murine leukemia virus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| APQVLPVMHP + H2O | - |
Moloney murine leukemia virus | APQVL + PVMHP | - |
? | |
| DELILPVKRK + H2O | - |
Moloney murine leukemia virus | DELIL + PVKRK | - |
? | |
| DSKAFLARDW + H2O | - |
Moloney murine leukemia virus | DSKAF + LARDW | - |
? | |
| DSKAFLATDW + H2O | - |
Moloney murine leukemia virus | DSKAF + LATDW | - |
? | |
| LTFTFPVVFMRR + H2O | - |
Moloney murine leukemia virus | LTFTF + PVVFMRR | - |
? | |
| MSKLLATVVS + H2O | - |
Moloney murine leukemia virus | MSKLL + ATVVS | - |
? | |
| PKDIFPVTET + H2O | - |
Moloney murine leukemia virus | PKDIF + PVTET | - |
? | |
| PLQVLTLNIERR + H2O | - |
Moloney murine leukemia virus | PLQVLTL + NIERR | - |
? | |
| PPAILPIISE + H2O | - |
Moloney murine leukemia virus | PPAIL + PIISE | - |
? | |
| PRSSLYPALRTP + H2O | - |
Moloney murine leukemia virus | PRSSLY + PALRTP | - |
? | |
| PRSSLYPALTP + H2O | - |
Moloney murine leukemia virus | PRSSLY + PALTP | - |
? | |
| PSEEYPIMID + H2O | - |
Moloney murine leukemia virus | PSEEY + PIMID | - |
? | |
| SDLVLLSAEARR + H2O | - |
Moloney murine leukemia virus | SDLVL + LSAEARR | - |
? | |
| TQTSLLTLDDQ + H2O | - |
Moloney murine leukemia virus | TQTSLL + TLDDQ | - |
? | |
| TQTSSLTLDDQ + H2O | - |
Moloney murine leukemia virus | TQTSSL + TLDDQ | - |
? | |
| TSQAFPLRAG + H2O | - |
Moloney murine leukemia virus | ? | - |
? | |
| TSQAFPLRAG + H2O | - |
Moloney murine leukemia virus | TSQAF + PLRAG | - |
? | |
| TSTLLIENSS + H2O | - |
Moloney murine leukemia virus | TSTLL + IENSS | - |
? | |
| VAQNYPIVQ-NH2 + H2O | - |
Moloney murine leukemia virus | VAQNY + PIVQ-NH2 | - |
? | |
| VFQNYPIVQ-NH2 + H2O | - |
Moloney murine leukemia virus | VFQNY + PIVQ-NH2 | - |
? | |
| VGFAGAMAEAE + H2O | - |
Moloney murine leukemia virus | VGFAG + AMAEAE | - |
? | |
| VGFAGAMAEAR + H2O | - |
Moloney murine leukemia virus | VGFAG + AMAEAR | - |
? | |
| VIQNYPIVQ-NH2 + H2O | - |
Moloney murine leukemia virus | VISQNY + PIVQ-NH2 | - |
? | |
| VKQNYPIVQ-NH2 + H2O | - |
Moloney murine leukemia virus | VKQNY + PIVQ-NH2 | - |
? | |
| VLQNYPIVQ-NH2 + H2O | - |
Moloney murine leukemia virus | VLQNY + PIVQ-NH2 | - |
? | |
| VMQNYPIVQ-NH2 + H2O | - |
Moloney murine leukemia virus | VMQNY + PIVQ-NH2 | - |
? | |
| VNQNYPIVQ-NH2 + H2O | - |
Moloney murine leukemia virus | VNQNY + PIVQ-NH2 | - |
? | |
| VQALVLTQ + H2O | - |
Moloney murine leukemia virus | VQAL + VLTQ | - |
? | |
| VSANYPIVQ-NH2 + H2O | - |
Moloney murine leukemia virus | VSANY + PIVQ-NH2 | - |
? | |
| VSDNYPIVQ-NH2 + H2O | - |
Moloney murine leukemia virus | VSDNY + PIVQ-NH2 | - |
? | |
| VSFNYPIVQ-NH2 + H2O | - |
Moloney murine leukemia virus | VSFNY + PIVQ-NH2 | - |
? | |
| VSGNYPIVQ-NH2 + H2O | - |
Moloney murine leukemia virus | VSGNY + PIVQ-NH2 | - |
? | |
| VSKNYPIVQ-NH2 + H2O | - |
Moloney murine leukemia virus | VSKNY + PIVQ-NH2 | - |
? | |
| VSLNYPIVQ-NH2 + H2O | - |
Moloney murine leukemia virus | VSLNY + PIVQ-NH2 | - |
? | |
| VSNNYPIVQ-NH2 + H2O | - |
Moloney murine leukemia virus | VSNNY + PIVQ-NH2 | - |
? | |
| VSQAYPIVQ-NH2 + H2O | - |
Moloney murine leukemia virus | VSQAY + PIVQ-NH2 | - |
? | |
| VSQDYPIVQ-NH2 + H2O | - |
Moloney murine leukemia virus | VSQDY + PIVQ-NH2 | - |
? | |
| VSQFYPIVQ-NH2 + H2O | - |
Moloney murine leukemia virus | VSQFY + PIVQ-NH2 | - |
? | |
| VSQGYPIVQ-NH2 + H2O | - |
Moloney murine leukemia virus | VSQGY + PIVQ-NH2 | - |
? | |
| VSQIYPIVQ-NH2 + H2O | - |
Moloney murine leukemia virus | VSQIY + PIVQ-NH2 | - |
? | |
| VSQLNYPIVQ-NH2 + H2O | - |
Moloney murine leukemia virus | VSQLY + PIVQ-NH2 | - |
? | |
| VSQNYPAVQ-NH2 + H2O | - |
Moloney murine leukemia virus | VSQNY + PAVQ-NH2 | - |
? | |
| VSQNYPFVQ-NH2 + H2O | - |
Moloney murine leukemia virus | VSQNY + PFVQ-NH2 | - |
? | |
| VSQNYPIAQ-NH2 + H2O | - |
Moloney murine leukemia virus | VSQNY + PIAQ-NH2 | - |
? | |
| VSQNYPIEQ-NH2 + H2O | - |
Moloney murine leukemia virus | VSQNY + PIWQ-NH2 | - |
? | |
| VSQNYPIFQ-NH2 + H2O | - |
Moloney murine leukemia virus | VSQNY + PIFQ-NH2 | - |
? | |
| VSQNYPIIQ-NH2 + H2O | - |
Moloney murine leukemia virus | VSQNY + PIIQ-NH2 | - |
? | |
| VSQNYPIKQ-NH2 + H2O | - |
Moloney murine leukemia virus | VSQNY + PIKQ-NH2 | - |
? | |
| VSQNYPILQ-NH2 + H2O | - |
Moloney murine leukemia virus | VSQNY + PILQ-NH2 | - |
? | |
| VSQNYPIQQ-NH2 + H2O | - |
Moloney murine leukemia virus | VSQNY + PIQQ-NH2 | - |
? | |
| VSQNYPIRQ-NH2 + H2O | - |
Moloney murine leukemia virus | VSQNY + PIRQ-NH2 | - |
? | |
| VSQNYPISQ-NH2 + H2O | - |
Moloney murine leukemia virus | VSQNY + PISQ-NH2 | - |
? | |
| VSQNYPIVQ + H2O | - |
Moloney murine leukemia virus | VSQNY + PIVQ | - |
? | |
| VSQNYPIVQ-NH2 + H2O | - |
Moloney murine leukemia virus | VSQNY + PIVQ-NH2 | - |
? | |
| VSQNYPLVQ-NH2 + H2O | - |
Moloney murine leukemia virus | VSQNY + PLVQ-NH2 | - |
? | |
| VSQNYPVVQ-NH2 + H2O | - |
Moloney murine leukemia virus | VSQNY + PVVQ-NH2 | - |
? | |
| VSQTYPIVQ-NH2 + H2O | - |
Moloney murine leukemia virus | VSQTY + PIVQ-NH2 | - |
? | |
| VSQVYPIVQ-NH2 + H2O | - |
Moloney murine leukemia virus | VSQVY + PIVQ-NH2 | - |
? | |
| VSVNYPIVQ-NH2 + H2O | - |
Moloney murine leukemia virus | VSVNY + PIVQ-NH2 | - |
? | |
| VTQNYPIVQ-NH2 + H2O | - |
Moloney murine leukemia virus | VTQNY + PIVQ-NH2 | - |
? | |
| VVQNYPIVQ-NH2 + H2O | - |
Moloney murine leukemia virus | VVQNY + PIVQ-NH2 | - |
? | |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.061 | - |
SDLVLLSAEARR | pH 6.0, 37°C | Moloney murine leukemia virus | |
| 0.07 | - |
PLQVLTLNIERR | pH 6.0, 37°C | Moloney murine leukemia virus | |
| 0.08 | - |
VGFAGAMAEAR | pH 6.0, 37°C | Moloney murine leukemia virus | |
| 0.0882 | - |
LTFTFPVVFMRR | pH 6.0, 37°C | Moloney murine leukemia virus | |
| 0.0963 | - |
APQVLPVMHP | pH 6.0, 37°C | Moloney murine leukemia virus | |
| 0.114 | - |
PPAILPIISE | pH 6.0, 37°C | Moloney murine leukemia virus | |
| 0.114 | - |
TQTSSLTLDDQ | pH 6.0, 37°C | Moloney murine leukemia virus | |
| 0.164 | - |
PRSSLYPALTP | pH 6.0, 37°C | Moloney murine leukemia virus | |
| 0.209 | - |
DELILPVKRK | pH 6.0, 37°C | Moloney murine leukemia virus | |
| 0.237 | - |
DSKAFLATDW | pH 6.0, 37°C | Moloney murine leukemia virus | |
| 0.279 | - |
VQALVLTQ | pH 6.0, 37°C | Moloney murine leukemia virus | |
| 0.294 | - |
TSTLLIENSS | pH 6.0, 37°C | Moloney murine leukemia virus | |
| 0.415 | - |
PSEEYPIMID | pH 6.0, 37°C | Moloney murine leukemia virus | |
| 0.439 | - |
MSKLLATVVS | pH 6.0, 37°C | Moloney murine leukemia virus | |
| 0.55 | - |
VSQNYPIVQ | pH 6.0, 37°C, wild-type enzyme | Moloney murine leukemia virus | |
| 0.714 | - |
TSQAFPLRAG | pH 6.0, 37°C | Moloney murine leukemia virus | |
| 1.27 | - |
PKDIFPVTET | pH 6.0, 37°C | Moloney murine leukemia virus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
recombinant enzyme | Moloney murine leukemia virus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4.3 | 7.5 | pH 4.3: about 60% of maximal activity, pH 7.5: about 40% of maximal activity, recombinant enzyme | Moloney murine leukemia virus |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 0.0022 | - |
IC50: 0.0022 mM | Moloney murine leukemia virus | UK88947 | |
| 0.025 | - |
IC50: 0.025 mM | Moloney murine leukemia virus | acetyl-pepstatin A | |
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