Literature summary for 3.4.23.B4 extracted from

Laco, G.S.; Fitzgerald, M.C.; Morris, G.M.; Olson, A.J.; Kent, S.B.; Elder, J.H.
Molecular analysis of the feline immunodeficiency virus protease: generation of a novel form of the protease by autoproteolysis and construction of cleavage-resistant proteases (1997), J. Virol., 71, 5505-5511.

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Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli, unit-length enzyme with the addition of a Met and Ala at the N-terminus. This results in an enzyme with only an additional Ala on the N-terminus with respect to the wild-type enzyme. The N-terminal Met is removed during expression presumably by an Escherichia coli methionine amino peptidase	feline immunodeficiency virus

Protein Variants

Protein Variants	Comment	Organism
D30N	mutant enzyme can bind substrate but is inactive	feline immunodeficiency virus
G5I/N55T	only 25% loss of activity after 16 h at pH 7.0, 37°C, in 0.2 M NaCl, compared to 65% loss of the wild-type enzyme	feline immunodeficiency virus
additional information	construction of cleavage-resistant mutants which have Km-values and turnover numbers similar to those of the wild-type enzyme	feline immunodeficiency virus
N55T	only 25% loss of activity after 16 h at pH 7.0, 37°C, in 0.2 M NaCl, compared to 65% loss of the wild-type enzyme	feline immunodeficiency virus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
additional information	feline immunodeficiency virus	the enzyme is essential for virion maturation and subsequent viral replication in that it cleaves the Gag and Gag/Pol polyproteins at eight sites to release the respective structural proteins and enzymes	?	-	?

Organism

Organism	UniProt	Comment	Textmining
feline immunodeficiency virus	-	-	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
proteolytic modification	the enzyme is expressed in vivo as part of the Gag/Pol polyprotein and, after autoprocessing out of the polyprotein, has a unit length of 116 amino acids, N-terminal Tyr, C-terminal Met	feline immunodeficiency virus

Purification (Commentary)

Purification (Comment)	Organism
during purification the recombinant enzyme underwent autoproteolysis to give discrete cleavage products. Construction of cleavage-resistant proteases which have Km and turnover-values similar to thoses of wild-type enzyme	feline immunodeficiency virus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	the enzyme is essential for virion maturation and subsequent viral replication in that it cleaves the Gag and Gag/Pol polyproteins at eight sites to release the respective structural proteins and enzymes	feline immunodeficiency virus	?	-	?

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
37	-	pH 7.0, 0.2 M NaCl, wild-type enzyme loses 65% of its activity	feline immunodeficiency virus

pH Stability

pH Stability	pH Stability Maximum	Comment	Organism
7	-	37°C, 0.2 M NaCl, wild-type enzyme loses 65% of ist activity	feline immunodeficiency virus

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Release 2023.1 (January 2023)