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Literature summary for 3.4.23.B3 extracted from
- Equine infectious anemia virus retropepsin (2004), Handbook of Proteolytic Enzymes (Barrett, J. ; Rawlings, N. D. ; Woessner, J. F. , eds. ), 1, 160-163.
No PubMed abstract available
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| the enzyme is encoded in the 5' part of the pol gene, expression as part of the Gag-Pol polyprotein precursor, expression in Escherichia coli | equine infectious anemia virus |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| crystal structure determination and analysis | equine infectious anemia virus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | an oligopeptide substrate representing the cleavage site between the matrix and capsid proteins of HIV-1 is highly active, while its P1'-pipecolic acid-substituted derivative is a specific inhibitor | equine infectious anemia virus |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| viral capsid | - |
equine infectious anemia virus | 19028 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 11431 | - |
2 * 10000-12000, SDS-PAGE, 2 * 11431, sequence calculation and mass spectrometry | equine infectious anemia virus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| EIAV Gag-Pol polypetide + H2O | equine infectious anemia virus | several cleavage sites | ? | - |
? |  |
| EIAV nucleocapsid protein + H2O | equine infectious anemia virus | processing in the early phase of virus replication | ? | - |
? | |
| additional information | equine infectious anemia virus | the retropepsin is expressed as part of the Gag-Pol polyprotein precursor, the enzyme cleaves the precursor into functional proteins during or after assembly and budding of the virions, thereby releasing itself | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| equine infectious anemia virus | - |
EIAV, a lenti-retrovirus, derived from horse, Equus caballus | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| proteolytic modification | the retropepsin is expressed as part of the Gag-Pol polyprotein precursor, the enzyme cleaves the precursor into functional proteins during or after assembly and budding of the virions, thereby releasing itself | equine infectious anemia virus |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme from virus grown in chronically infected dog thymus cells, ATCC CRL 1430, recombinant enzyme from Escherichia coli by several different steps of ion exchange chromatography | equine infectious anemia virus |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| Processing at the authentic HIV-1 PR recognition site and release of the mature p17 matrix and the p24 capsid protein, as a result of the cleavage of the -SQNY-/-PIVQ- cleavage site. | substrate binding sites and mechanism, characterization by type 1 substrates, overview | equine infectious anemia virus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| EIAV Gag-Pol polypeptide + H2O | several cleavage sites | equine infectious anemia virus | ? | - |
? | |
| EIAV Gag-Pol polypetide + H2O | several cleavage sites | equine infectious anemia virus | ? | - |
? | |
| EIAV nucleocapsid protein + H2O | - |
equine infectious anemia virus | ? | - |
? | |
| EIAV nucleocapsid protein + H2O | processing in the early phase of virus replication | equine infectious anemia virus | ? | - |
? | |
| HIV-1 Gag-Pol polypeptide + H2O | low activity | equine infectious anemia virus | ? | - |
? | |
| additional information | the retropepsin is expressed as part of the Gag-Pol polyprotein precursor, the enzyme cleaves the precursor into functional proteins during or after assembly and budding of the virions, thereby releasing itself | equine infectious anemia virus | ? | - |
? | |
| additional information | an oligopeptide substrate representing the cleavage site between the matrix and capsid proteins of HIV-1 is highly active, while its P1'-pipecolic acid-substituted derivative is a specific inhibitor | equine infectious anemia virus | ? | - |
? | |
| Val-Ser-Gln-Asn-Tyr-Pro-Ile-Val-Gln + H2O | commercial substrate | equine infectious anemia virus | Val-Ser-Gln-Asn-Tyr + Pro-Ile-Val-Gln | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 10000-12000, SDS-PAGE, 2 * 11431, sequence calculation and mass spectrometry | equine infectious anemia virus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| EIAV retropepsin | - |
equine infectious anemia virus |
| equine infectious anemia virus retropepsin | - |
equine infectious anemia virus |
| More | the enzyme belongs to the A2 peptidase family | equine infectious anemia virus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 6 | activity range with peptide substrates in presence of NaCl | equine infectious anemia virus |
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