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Literature summary for 3.4.23.B24 extracted from

  • Bat-Ochir, C.; Kwak, J.Y.; Koh, S.K.; Jeon, M.H.; Chung, D.; Lee, Y.W.; Chae, S.K.
    The signal peptide peptidase SppA is involved in sterol regulatory element-binding protein cleavage and hypoxia adaptation in Aspergillus nidulans (2016), Mol. Microbiol., 100, 635-655 .
    View publication on PubMed
Search for more literature for 3.4.23.B24

Cloned(Commentary)

Cloned (Comment) Organism
gene sppA, phylogenetic analysis, recombinant expression of FLAG-tagged wild-type enzyme SppA and mutant D337A SppA in the Aspergillus nidulans DELTAsppA knockout mutant strain, recombinant expression of N-terminally YFP-tagged enzyme in wild-type Aspergillus nidulans Aspergillus nidulans

Protein Variants

Protein Variants Comment Organism
D337A site-directed mutagenesis, inactive mutant Aspergillus nidulans
additional information generation of a knockout enzyme null mutant DELTAsppA Aspergillus nidulans

Localization

Localization Comment Organism GeneOntology No. Textmining
endoplasmic reticulum membrane SppA interacts with the sterol regulatory element-binding protein SrbA in the endoplasmic reticulum, where SppA localizes in normoxia and hypoxia Aspergillus nidulans 5789
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Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
sterol regulatory element-binding protein + H2O Aspergillus nidulans SrbA is sequentially cleaved by Dsc complex-linked proteolysis and SppA ?
-
 ?
sterol regulatory element-binding protein + H2O Aspergillus nidulans A773 SrbA is sequentially cleaved by Dsc complex-linked proteolysis and SppA ?
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 ?

Organism

Organism UniProt Comment Textmining
Aspergillus nidulans
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 i.e. Aspergillus nidulans
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Aspergillus nidulans A773
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 i.e. Aspergillus nidulans
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sterol regulatory element-binding protein + H2O
-
 Aspergillus nidulans ?
-
 ?
sterol regulatory element-binding protein + H2O SrbA is sequentially cleaved by Dsc complex-linked proteolysis and SppA Aspergillus nidulans ?
-
 ?
sterol regulatory element-binding protein + H2O
-
 Aspergillus nidulans A773 ?
-
 ?
sterol regulatory element-binding protein + H2O SrbA is sequentially cleaved by Dsc complex-linked proteolysis and SppA Aspergillus nidulans A773 ?
-
 ?

Synonyms

Synonyms Comment Organism
ANID_08681 locus name Aspergillus nidulans
SppA
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 Aspergillus nidulans

General Information

General Information Comment Organism
malfunction both null and dead activity mutants of sppA fail to grow in hypoxia, and the growth defect of DELTAsppA is complemented by nuclear SrbA-N381 expression. The loss of SppA or Dsc orthologs leads to the mislocalization of sterol regulatory element-binding protein SrbA within the cell. Expression of the truncated SrbA-N414 covering the SrbA sequence prior to the second transmembrane region rescues the growth of DELTAdscA but not of DELTAsppA in hypoxia Aspergillus nidulans
physiological function the signal peptide peptidase (SPP) SppA, an aspartyl protease involved in regulated intramembrane proteolysis (RIP), is essential for hypoxia adaptation in Aspergillus nidulans. Importance of SppA and the Dsc complex for nuclear localization of sterol regulatory element-binding protein (SREBP) SrbA in hypoxia. Sequential cleavage of SrbA by Dsc-linked proteolysis followed by SppA, proposing another model of RIP for sterol regulatory element-binding protein (SREBP) srbA cleavage in fungal hypoxia adaptation Aspergillus nidulans