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Literature summary for 3.4.23.B24 extracted from
- A novel strategy to improve protein secretion via overexpression of the SppA signal peptide peptidase in Bacillus licheniformis (2017), Microb. Cell Fact., 16, 70 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| enzyme overexpression in Bacillus licheniformis, gene sppA is integrated into the genome | Bacillus licheniformis |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | improvement of protein secretion via overexpression of the SppA signal peptide peptidase in Bacillus licheniformis. Enzyme knockout of gene sppA, encoding the signal peptide peptidase, individually in Bacillus licheniformis strain BL10. Overexpression of sppA could not only improve the protein secretion level, but also enhance the biomass yield | Bacillus licheniformis |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | signal peptide peptidase (SPP) is a membrane-bound enzyme | Bacillus licheniformis | 16020 | - |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| alpha-amylase + H2O | Bacillus licheniformis | - |
? | - |
? |  |
| alpha-amylase + H2O | Bacillus licheniformis BL10 | - |
? | - |
? | |
| nattokinase + H2O | Bacillus licheniformis | - |
? | - |
? | |
| nattokinase + H2O | Bacillus licheniformis BL10 | - |
? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus licheniformis | - |
- |
- |
| Bacillus licheniformis BL10 | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| alpha-amylase + H2O | - |
Bacillus licheniformis | ? | - |
? | |
| alpha-amylase + H2O | - |
Bacillus licheniformis BL10 | ? | - |
? | |
| nattokinase + H2O | - |
Bacillus licheniformis | ? | - |
? | |
| nattokinase + H2O | - |
Bacillus licheniformis BL10 | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| SppA | - |
Bacillus licheniformis |
General Information
| General Information | Comment | Organism |
|---|---|---|
| malfunction | dramatic decreases in the concentrations of both signal peptide peptidases SppA and TepA (45 and 49%, respectively) in a sppA deficient strain, while the extracellular protein yields of nattokinase and alpha-amylase are increased by 30% and 67% respectively in a strain overexpressing SppA. In addition, biomass, specific enzyme activities and the relative gene transcriptional levels are also enhanced due to the overexpression of sppA, while altering the expression levels of tepA has no effect on the concentrations of the secreted target proteins | Bacillus licheniformis |
| physiological function | signal peptide peptidase (SPP) uses a serine/lysine catalytic dyad mechanism to cleave the remnant signal peptides in the cellular membrane and aids in protein secretion | Bacillus licheniformis |
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Release 2023.1 (January 2023)
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