Literature summary for 3.4.23.B24 extracted from

Nam, S.E.; Kim, A.C.; Paetzel, M.
Crystal structure of Bacillus subtilis signal peptide peptidase A (2012), J. Mol. Biol., 419, 347-358.

Search for more literature for 3.4.23.B24

Crystallization (Commentary)

Crystallization (Comment)	Organism
2.4-A resolution structure of Bacillus subtilis SppA catalytic domain reveals eight SppA molecules in the asymmetric unit, forming a dome-shaped octameric complex. Residue Ser147 may serve as the nucleophile and Lys199 may serve as the general base. The SppA S1 substrate specificity pocket is deep, narrow and hydrophobic, but with a polar bottom. The S3 pocket, which is constructed from two neighboring proteins, is shallower, wider and more polar than the S1 pocket	Bacillus subtilis

Crystallization (Comment)

Organism

2.4-A resolution structure of Bacillus subtilis SppA catalytic domain reveals eight SppA molecules in the asymmetric unit, forming a dome-shaped octameric complex. Residue Ser147 may serve as the nucleophile and Lys199 may serve as the general base. The SppA S1 substrate specificity pocket is deep, narrow and hydrophobic, but with a polar bottom. The S3 pocket, which is constructed from two neighboring proteins, is shallower, wider and more polar than the S1 pocket

Bacillus subtilis

Protein Variants

Protein Variants	Comment	Organism
K199A	inactive	Bacillus subtilis
S147A	inactive	Bacillus subtilis

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
28000	-	8 * 36000, SDS-PAGE. 8 * 28000, SDS-PAGE after limited digestion using thermolysin	Bacillus subtilis
36000	-	8 * 36000, SDS-PAGE. 8 * 28000, SDS-PAGE after limited digestion using thermolysin	Bacillus subtilis
225400	-	gel filtration	Bacillus subtilis

Organism

Organism	UniProt	Comment	Textmining
Bacillus subtilis	O34525	-	-
Bacillus subtilis 168	O34525	-	-

Subunits

Subunits	Comment	Organism
octamer	8 * 36000, SDS-PAGE. 8 * 28000, SDS-PAGE after limited digestion using thermolysin	Bacillus subtilis

Synonyms

Synonyms	Comment	Organism
SppA	-	Bacillus subtilis

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Release 2023.1 (January 2023)