Literature summary for 3.4.23.B24 extracted from

Nam, S.E.; Paetzel, M.
Structure of signal peptide peptidase A with C-termini bound in the active sites: insights into specificity, self-processing, and regulation (2013), Biochemistry, 52, 8811-8822.

Search for more literature for 3.4.23.B24

Cloned(Commentary)

Cloned (Comment)	Organism
-	Bacillus subtilis

Crystallization (Commentary)

Crystallization (Comment)	Organism
crystal structure of a proteolytically stable fragment of mutant K199A, residues Leu51-Gly295, that has its C-terminal peptide bound in each of the eight active sites, creating a perfect circle of peptides. Substrate specificity pockets accommodate C-terminal residues Tyr331, Met329, and Tyr333, respectively. The C-terminus binds within the substrate-binding grooves in an antiparallel beta-sheet fashion	Bacillus subtilis

Crystallization (Comment)

Organism

crystal structure of a proteolytically stable fragment of mutant K199A, residues Leu51-Gly295, that has its C-terminal peptide bound in each of the eight active sites, creating a perfect circle of peptides. Substrate specificity pockets accommodate C-terminal residues Tyr331, Met329, and Tyr333, respectively. The C-terminus binds within the substrate-binding grooves in an antiparallel beta-sheet fashion

Bacillus subtilis

Protein Variants

Protein Variants	Comment	Organism
K199A	active site mutant	Bacillus subtilis
additional information	construction of C-terminal deletion mutants DELTA329-335, DELTA307-335 and DELTA295-335. The C-terminus is not essential for oligomerization of the enzyme	Bacillus subtilis

Inhibitors

Inhibitors	Comment	Organism	Structure
NH3-SPRMMYLYAK-COOH	peptide synthesized corresponding to the sequence of the modeled C-terminal peptide bound in the SppA substrate-binding groove. Competitive inhibition	Bacillus subtilis

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0179	-	dodecanoyl-NGEVAKA-4-methylcumaryl-7-amide	pH 8.0, 23°C	Bacillus subtilis

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
26624	-	x * 26624, electrospray ionization mass spectrometry of protease resistant fragment Leu51-Gly295	Bacillus subtilis

Organism

Organism	UniProt	Comment	Textmining
Bacillus subtilis	O34525	-	-
Bacillus subtilis 168	O34525	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
dodecanoyl-NGEVAKA-4-methylcumaryl-7-amide + H2O	-	Bacillus subtilis	?	-	?
dodecanoyl-NGEVAKA-4-methylcumaryl-7-amide + H2O	-	Bacillus subtilis 168	?	-	?
additional information	peptidase SppABS self-processes its own C-terminus. The processing occurs very quickly and is complete before the enzyme can be purified. No trans-processing is observed between wild-type and active site mutants	Bacillus subtilis	?	-	?
additional information	peptidase SppABS self-processes its own C-terminus. The processing occurs very quickly and is complete before the enzyme can be purified. No trans-processing is observed between wild-type and active site mutants	Bacillus subtilis 168	?	-	?

Subunits

Subunits	Comment	Organism
?	x * 26624, electrospray ionization mass spectrometry of protease resistant fragment Leu51-Gly295	Bacillus subtilis

Synonyms

Synonyms	Comment	Organism
SppA	-	Bacillus subtilis

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.078	-	dodecanoyl-NGEVAKA-4-methylcumaryl-7-amide	pH 8.0, 23°C	Bacillus subtilis

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.0021	-	NH3-SPRMMYLYAK-COOH	pH 8.0, 23°C	Bacillus subtilis