Literature summary for 3.4.23.B2 extracted from

Grant, S.K.; Deckman, I.C.; Culp, J.S.; Minnich, M.D.; Brooks, I.S.; Hensley, P.; Debouck, C.; Meek, T.D.
Use of protein unfolding studies to determine the conformational and dimeric stabilities of HIV-1 and SIV proteases (1992), Biochemistry, 31, 9491-9501.

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General Stability

General Stability	Organism
8 M urea cause 50% quenching of the fluorescence emission	Simian immunodeficiency virus

Inhibitors

Inhibitors	Comment	Organism	Structure
Ala-Ala-Phe-Psi[CH(OH)CH2]Gly-Val-ValOCH3	-	Simian immunodeficiency virus

Organism

Organism	UniProt	Comment	Textmining
Simian immunodeficiency virus	-	-	-

Renatured (Commentary)

Renatured (Comment)	Organism
nearly total restoration of protein fluorescence upon cooling to 25°C of the enzyme which has been unfolded at 70°C	Simian immunodeficiency virus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
Ac-Arg-Ala-Ser-Gln-Asn-Tyr-Pro-Val-Val-NH2 + H2O	-	Simian immunodeficiency virus	Ac-Arg-Ala-Ser-Gln-Asn-Tyr-OH + Pro-Val-Val-NH2	-	?

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
40	-	-	Simian immunodeficiency virus

Temperature Range [°C]

Temperature Minimum [°C]	Temperature Maximum [°C]	Comment	Organism
20	50	20°C: 60% of maximal activity, 50°C: about 80% of maximal activity	Simian immunodeficiency virus

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
70	-	thermal denaturation	Simian immunodeficiency virus

pH Stability

pH Stability	pH Stability Maximum	Comment	Organism
3	6	60 min, stable	Simian immunodeficiency virus
7	8	activity is slightly diminished, less than 30%, after preincubation at pH 7.0-8.0	Simian immunodeficiency virus

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.000012	-	Ala-Ala-Phe-Psi[CH(OH)CH2]Gly-Val-ValOCH3	pH 4.7, 37°C	Simian immunodeficiency virus

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Release 2023.1 (January 2023)