Crystallization (Comment) | Organism |
---|---|
hanging-drop crystallization of the K69H mutant enzyme with pepstatin A | Simian immunodeficiency virus |
Protein Variants | Comment | Organism |
---|---|---|
D25N | totally inactive mutant enzyme, incapable of autoprocessing | Simian immunodeficiency virus |
K69H | the isoelectric point of the mutant enzyme is pH 7.0 compared to that of the wild-type enzyme of 7.4. The purified mutant enzyme is less soluble at low pH when concentrating sample in preparation for crystallization | Simian immunodeficiency virus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
pepstatin A | - |
Simian immunodeficiency virus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Simian immunodeficiency virus | - |
wild-type and mutant enzymes K69H and D25N | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Lys-Ala-Arg-Val-Nle-(NO2)Phe-Glu-Ala-Nle-Gly + H2O | - |
Simian immunodeficiency virus | Lys-Ala-Arg-Val-Nle + (NO2)Phe-Glu-Ala-Nle-Gly | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | 5.5 | - |
Simian immunodeficiency virus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Simian immunodeficiency virus | mutant enzyme K69H | - |
7 |