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Literature summary for 3.4.23.52 extracted from

  • Henche, A.L.; van Wolferen, M.; Ghosh, A.; Albers, S.V.
    Dissection of key determinants of cleavage activity in signal peptidase III (SPaseIII) PibD (2014), Extremophiles, 18, 905-913.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Sulfolobus acidocaldarius

Protein Variants

Protein Variants Comment Organism
A77E mutant enzyme shows no processing of prepillin AapA Sulfolobus acidocaldarius
A77G activity level is comparable to that of the wild type enzyme under standard assay conditions Sulfolobus acidocaldarius
A77P activity level is comparable to that of the wild type enzyme under standard assay conditions Sulfolobus acidocaldarius
A77P cleavage activity is reduced Sulfolobus acidocaldarius
D185A activity of the mutant enzyme is lower than activity of wild-type enzyme Sulfolobus acidocaldarius
D186A activity level is comparable to that of the wild type enzyme under standard assay conditions Sulfolobus acidocaldarius
D21A mutant enzyme shows no processing of prepillin AapA Sulfolobus acidocaldarius
D78A mutant enzyme shows no processing of prepillin AapA Sulfolobus acidocaldarius
G75E mutant enzyme shows no processing of prepillin AapA Sulfolobus acidocaldarius
I22E activity level is comparable to that of the wild type enzyme under standard assay conditions Sulfolobus acidocaldarius
K23A activity level is comparable to that of the wild type enzyme under standard assay conditions Sulfolobus acidocaldarius
K24A activity level is comparable to that of the wild type enzyme under standard assay conditions Sulfolobus acidocaldarius
L81A activity level is comparable to that of the wild type enzyme under standard assay conditions Sulfolobus acidocaldarius
R25A single mutant displays Aap pili even though no preopillin AapA-processing is observed Sulfolobus acidocaldarius
W32A mutation results in reduced activity Sulfolobus acidocaldarius

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
28000
-
-
Sulfolobus acidocaldarius

Organism

Organism UniProt Comment Textmining
Sulfolobus acidocaldarius Q4JCB7
-
-
Sulfolobus acidocaldarius DSM 639 Q4JCB7
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
prepilin AapA + H2O
-
Sulfolobus acidocaldarius ?
-
?
prepilin AapA + H2O
-
Sulfolobus acidocaldarius DSM 639 ?
-
?

Synonyms

Synonyms Comment Organism
PibD
-
Sulfolobus acidocaldarius
saci_0139 locus name Sulfolobus acidocaldarius
signal peptidase III
-
Sulfolobus acidocaldarius
SPaseIII
-
Sulfolobus acidocaldarius