Literature summary for 3.4.23.5 extracted from

Buckow, R.; Truong, B.; Versteeg, C.
Bovine cathepsin D activity under high pressure (2010), Food Chem., 120, 474-481.

General Stability

General Stability	Organism
the protease is largely pressure stable at room temperature. At 100 MPa a significant stabilisation of the enzyme against temperature-induced inactivation is observed. Pressure drastically inhibits the cleavage by cathepsin D in Bis-Tris buffer (pH 6.0) causing a reduction of the catalytic rate of more than 50% at 100-400 MPa. The enzyme is relatively stable with more than 57% and 40% residual activity after 30 min treatment at 30°C and 300 MPa or 400 MPa, respectively. At higher pressures the enzyme is destabilised rapidly resulting in a residual activity of less than 10% after 30 min treatment at 600 MPa	Bos taurus

Organism

the protease is largely pressure stable at room temperature. At 100 MPa a significant stabilisation of the enzyme against temperature-induced inactivation is observed. Pressure drastically inhibits the cleavage by cathepsin D in Bis-Tris buffer (pH 6.0) causing a reduction of the catalytic rate of more than 50% at 100-400 MPa. The enzyme is relatively stable with more than 57% and 40% residual activity after 30 min treatment at 30°C and 300 MPa or 400 MPa, respectively. At higher pressures the enzyme is destabilised rapidly resulting in a residual activity of less than 10% after 30 min treatment at 600 MPa

Bos taurus

Organism

Organism	UniProt	Comment	Textmining
Bos taurus	-	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
spleen	-	Bos taurus	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
Hemoglobin + H2O	-	Bos taurus	?	-	?
Myoglobin + H2O	the hydrolysis rate is fairly slow	Bos taurus	?	-	?

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
60	-	maximal substrate cleavage by cathepsin D is identified at 60°C and ambient pressure conditions after 20 min treatment	Bos taurus

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
25	60	the protease is heat stable at ambient pressure up to 300 MPa and 55°C, respectively, causing less than 10% inactivation after 10 min treatment. Cathepsin D is quickly inactivated at temperatures above 60°C	Bos taurus