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Literature summary for 3.4.23.5 extracted from

  • Minarowska, A.; Karwowska, A.; Gacko, M.
    Quantitative determination and localization of cathepsin D and its inhibitors (2009), Folia Histochem. Cytobiol., 47, 153-177.
    View publication on PubMed
Search for more literature for 3.4.23.5

Inhibitors

Inhibitors Comment Organism Structure
additional information cathepsin D has neither endogenous lysosomal nor cytosolic inhibitors Rattus norvegicus
pepstatin A
-
 Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
Golgi apparatus procathepsin D is transported from cisterns of the rough endoplasmic reticulum to the Golgi apparatus Rattus norvegicus 5794
-
lysosome
-
 Rattus norvegicus 5764
-
rough endoplasmic reticulum cathepsin D is synthesized in the rough endoplasmic reticulum as preprocathepsin D Rattus norvegicus 5791
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
14000
-
 1 * 34000 + 1 * 14000, mature cathepsin D Rattus norvegicus
34000
-
 1 * 34000 + 1 * 14000, mature cathepsin D Rattus norvegicus
48000
-
 single chain intermediate cathepsin D Rattus norvegicus
52000
-
 pro-cathepsin D Rattus norvegicus
54000
-
 prepro-cathepsin D Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Albumin + H2O Rattus norvegicus 15% cleavage at pH 3.0 ?
-
 ?
alpha-globulin + H2O Rattus norvegicus 12% cleavage at pH 3.0 and 3.5 ?
-
 ?
beta-globulin + H2O Rattus norvegicus 10% cleavage at pH 3.0 and 3.5 ?
-
 ?
casein + H2O Rattus norvegicus 20% cleavage at pH 3.5 ?
-
 ?
Fibrin + H2O Rattus norvegicus 12% cleavage at pH 3.0 ?
-
 ?
Hemoglobin + H2O Rattus norvegicus 100% cleavage at pH 3.0 ?
-
 ?
additional information Rattus norvegicus does not cleave gamma-globulin and fibrinogen ?
-
 ?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
adrenal gland highest hydrolytic activity Rattus norvegicus
-
blood plasma
-
 Rattus norvegicus
-
bone marrow
-
 Rattus norvegicus
-
cerebellum
-
 Rattus norvegicus
-
cerebrum
-
 Rattus norvegicus
-
colon
-
 Rattus norvegicus
-
duodenum
-
 Rattus norvegicus
-
heart
-
 Rattus norvegicus
-
ileum
-
 Rattus norvegicus
-
jejunum
-
 Rattus norvegicus
-
kidney
-
 Rattus norvegicus
-
Kupffer cell Kupffer cells exhibit highest liver cathepsin D activity Rattus norvegicus
-
liver
-
 Rattus norvegicus
-
lung
-
 Rattus norvegicus
-
lymph node
-
 Rattus norvegicus
-
additional information very low detection in serum and blood cells Rattus norvegicus
-
muscle
-
 Rattus norvegicus
-
pancreas
-
 Rattus norvegicus
-
spleen
-
 Rattus norvegicus
-
stomach
-
 Rattus norvegicus
-
thymus
-
 Rattus norvegicus
-
urinary bladder
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Albumin + H2O 15% cleavage at pH 3.0 Rattus norvegicus ?
-
 ?
alpha-globulin + H2O 12% cleavage at pH 3.0 and 3.5 Rattus norvegicus ?
-
 ?
AMCA-Glu-Glu-L-Lys-L-Pro-L-Ile-L-Ser-L-Phe-L-Phe-L-Arg-L-Leu-Gly-L-Lys(biotinyl)-NH2 + H2O
-
 Rattus norvegicus AMCA-Glu-Glu-L-Lys-L-Pro-L-Ile-L-Ser-L-Phe + L-Phe-L-Arg-L-Leu-Gly-L-Lys(biotinyl)-NH2
-
 ?
benzoyl-L-Arg-Gly-L-Phe-L-Phe-L-Leu-4-methoxy-2-naphthylamide + H2O
-
 Rattus norvegicus ?
-
 ?
benzoyl-L-Arg-L-Pro-L-Phe-L-Phe-L-Leu-4-methoxy-2-naphthylamide + H2O
-
 Rattus norvegicus ?
-
 ?
benzyloxycarbonyl-L-Pro-L-Phe-L-His-L-Leu-L-Leu-L-Val-L-Tyr-L-Ser-2-naphthylamide + H2O
-
 Rattus norvegicus ?
-
 ?
beta-globulin + H2O 10% cleavage at pH 3.0 and 3.5 Rattus norvegicus ?
-
 ?
casein + H2O 20% cleavage at pH 3.5 Rattus norvegicus ?
-
 ?
D-Phe-L-Ser-L-Phe-L-Phe-L-Ala-L-Ala-4-aminobenzoate + H2O
-
 Rattus norvegicus D-Phe-L-Ser-L-Phe + L-Phe-L-Ala-L-Ala-4-aminobenzoate
-
 ?
D-Phe-L-Ser-L-Ser-L-Phe-L-Phe-L-Ala-4-aminobenzoate + H2O
-
 Rattus norvegicus ?
-
 ?
Fibrin + H2O 12% cleavage at pH 3.0 Rattus norvegicus ?
-
 ?
Gly-L-Phe-L-Leu-Gly-D-Phe-L-Leu + H2O 3.0% cleavage Rattus norvegicus Gly-L-Phe-L-Leu-Gly + D-Phe-L-Leu
-
 ?
Gly-L-Phe-L-Leu-Gly-L-Phe-D-Leu + H2O 6.0% cleavage Rattus norvegicus Gly-L-Phe-L-Leu-Gly + L-Phe-D-Leu
-
 ?
Gly-L-Phe-L-Leu-Gly-L-Phe-L-Leu + H2O 100% cleavage Rattus norvegicus Gly-L-Phe-L-Leu-Gly + L-Phe-L-Leu
-
 ?
Hemoglobin + H2O 100% cleavage at pH 3.0 Rattus norvegicus ?
-
 ?
L-Asp-L-Arg-L-Val-L-Tyr-L-Ile-L-His-L-Pro-L-Phe-L-His-L-Leu-L-Leu-L-Val-L-Tyr-L-Ser-OH + H2O
-
 Rattus norvegicus ?
-
 ?
L-Asp-L-Arg-L-Val-L-Tyr-L-Ile-L-His-L-Pro-L-Phe-L-His-L-Leu-L-Val-L-Ile-L-His-OH + H2O
-
 Rattus norvegicus ?
-
 ?
L-Asp-L-Val-L-Arg-L-Tyr-L-Ile-L-His-L-Pro-L-Phe-L-His-L-Leu-L-Leu-L-Val-L-Tyr-L-Ser-OH + H2O
-
 Rattus norvegicus ?
-
 ?
L-Phe-L-Ala-L-Ala-L-Phe(NO2)-L-Phe-L-Val-L-Leu-4-hydroxymethyl(pyridine) + H2O
-
 Rattus norvegicus L-Phe-L-Ala-L-Ala-L-Phe(NO2) + L-Phe-L-Val-L-Leu-4-hydroxymethyl(pyridine)
-
 ?
additional information does not cleave gamma-globulin and fibrinogen Rattus norvegicus ?
-
 ?
additional information does not cleave Gly-D-Phe-L-Leu-Gly-L-Phe-L-Leu, Gly-D-Phe-L-Leu-Gly-D-Phe-L-Leu, and cyclic Gly-L-Phe-L-Leu-Gly-L-Phe-L-Leu Rattus norvegicus ?
-
 ?
N-acetyl-Gly-L-Phe-L-Leu-Gly-L-Phe-OH + H2O
-
 Rattus norvegicus ?
-
 ?

Subunits

Subunits Comment Organism
heterodimer 1 * 34000 + 1 * 14000, mature cathepsin D Rattus norvegicus

Synonyms

Synonyms Comment Organism
CTSD
-
 Rattus norvegicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Rattus norvegicus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 50 at 20°C: about 70% of maximum activity, at 50°C: about 30% of maximum activity Rattus norvegicus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
20 65 completely stable between 20 and 40°C, thereafter activity decreases continuously, 20% loss of activity at 50°C, 80% loss of activity at 60°C, complete inactivation at 65°C Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
3
-
 complexification of hemoglobin with haptoglobin reduces the optimum pH from 3.0 to 2.8 for degradation and simultaneously causes a 50% decrease in degradation rate 50% Rattus norvegicus

pH Range

pH Minimum pH Maximum Comment Organism
3 5 at pH 3.0: about 25% of maximum activity, at pH 5.0: about 45% of maximum activity, beta-endorphin hydrolysis Rattus norvegicus

pH Stability

pH Stability pH Stability Maximum Comment Organism
2.5 8 completely stable at pH 4.5-5.5, 90% loss of activity at pH 2.5, about 40% loss of activity at pH 3.0, about 5% loss of activity at pH 3.5-4.0, about 10% loss of activity at pH 6.0, about 30% loss of activity at pH 7.0, about 50% loss of activity at pH 7.5, about 80% loss of activity at pH 8.0 Rattus norvegicus

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.00001
-
 IC50 less than 0.00001 mM, pH and temperature not specified in the publication Rattus norvegicus pepstatin A