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Literature summary for 3.4.23.5 extracted from

  • Morales, M.E.; Rinaldi, G.; Gobert, G.N.; Kines, K.J.; Tort, J.F.; Brindley, P.J.
    RNA interference of Schistosoma mansoni cathepsin D, the apical enzyme of the hemoglobin proteolysis cascade (2008), Mol. Biochem. Parasitol., 157, 160-168.
    View publication on PubMedView publication on EuropePMC

Protein Variants

Protein Variants Comment Organism
additional information cultured schistosomules of Schistosoma mansoni are exposed by square wave electroporation to double stranded RNA specific for cDNA encoding cathepsin D. RNAi-mediated reductions in transcript levels leads to phenotypic changes including significant growth retardation in vitro and suppression of aspartic protease enzyme activity. In addition, black-pigmented heme, the end point by-product of normal hemoglobin proteolysis that accumulates in the schistosome gut, is not apparent within the guts of the treated schistosomules. Their guts appear to be red in color, rather than black, apparently indicating the presence of intact rather than digested host hemoglobin Schistosoma mansoni

Organism

Organism UniProt Comment Textmining
Schistosoma mansoni
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7-methoxycoumarin-4-acetyl-Gly-Lys-Pro-Ile-Leu-Phe-Phe-Arg-Leu-Lys(DNP)-Arg-amide + H2O
-
Schistosoma mansoni ?
-
?

Synonyms

Synonyms Comment Organism
cathepsin D
-
Schistosoma mansoni

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Schistosoma mansoni

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5
-
assay at Schistosoma mansoni